| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, GIT1 |
| Entrez GeneID | 28964 |
| WB Predicted band size | 84.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GIT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y554 of human GIT1 (Q9Y2X7-1). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-GIT1(Y554)抗体的参考文献示例(注：部分文献为模拟示例，实际引用时请核实来源)：
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1. **"Phosphorylation of GIT1 at Tyr-554 Promotes Src-Induced Podosome Formation via Interaction with paxillin"**
*Authors: Zhao Z, et al.*
**摘要**：研究GIT1在Y554位点的磷酸化如何通过Src激酶调控其与paxillin的相互作用，从而促进细胞伪足形成。该文献使用Phospho-GIT1(Y554)抗体验证了磷酸化事件在细胞迁移中的作用。
2. **"Tyrosine Phosphorylation of GIT1 Modulates Its Scaffolding Function in Focal Adhesion Dynamics"**
*Authors: Kim S, et al.*
**摘要**：探讨GIT1的Y554磷酸化对其支架功能的调控，证明该修饰通过影响βPIX复合物的募集，调节黏着斑的稳定性。抗体用于Western blot和免疫荧光定位。
3. **"GIT1 Phosphorylation by c-Abl Regulates Neuronal Branching and Synaptic Plasticity"**
*Authors: Wang L, et al.*
**摘要**：发现c-Abl激酶介导的GIT1-Y554磷酸化在神经元树突分支和突触可塑性中的关键作用，通过特异性抗体揭示磷酸化水平与神经元发育的相关性。
4. **"Role of GIT1 Tyrosine 554 Phosphorylation in Tumor Angiogenesis"**
*Authors: Chen X, et al.*
**摘要**：分析GIT1-Y554磷酸化在肿瘤血管生成中的功能，证明其通过VEGF信号通路调控内皮细胞迁移，抗体用于组织样本的磷酸化检测。
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**说明**：以上文献为示例性质，实际研究中建议通过PubMed、Google Scholar等数据库，以关键词“Phospho-GIT1(Y554)”或“GIT1 phosphorylation Tyr554”检索最新文献，并核实抗体生产商(如CST、Abcam)提供的引用文献列表。
Phospho-GIT1(Y554) antibody is a specialized tool used to detect the phosphorylated form of GIT1 (G protein-coupled receptor kinase-interacting protein 1) at tyrosine residue 554. GIT1 is a multifunctional scaffolding protein involved in regulating intracellular signaling, cell adhesion, and cytoskeletal dynamics. It interacts with various partners, including PAK kinases, PIX proteins, and focal adhesion components, playing roles in cell migration, vesicle trafficking, and synaptic plasticity. Phosphorylation at Y554 is a critical post-translational modification linked to GIT1’s activation and function. This site is targeted by Src family kinases, which phosphorylate Y554 in response to extracellular stimuli like growth factors or integrin engagement. The modification enhances GIT1’s ability to recruit downstream effectors, influencing pathways such as MAPK/ERK signaling and focal adhesion turnover.
The Phospho-GIT1(Y554) antibody is widely used in research to study cellular processes like cancer metastasis, neuronal development, and cardiovascular remodeling. It helps identify phosphorylation-dependent GIT1 activity in disease models, particularly in cancers with dysregulated tyrosine kinase signaling or neurological disorders involving synaptic dysfunction. Researchers validate its specificity using knockout controls or phosphatase treatment. Understanding GIT1 phosphorylation provides insights into mechanisms of cell motility, proliferation, and tissue organization, making this antibody valuable for both basic and translational studies.
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