首页 / 产品 / 抗体 / 一抗

Rabbit Polyclonal Phospho-RAF1(Y340) Antibody

  • 中文名： Phospho-RAF1(Y340)抗体
  • 别    名： RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF
货号: IPDX30301
Price： ￥1280
数量：
大包装询价

验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAQP0; LIM1; MP26; MIP26; CTRCT15
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human MIP
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

+ +

参考文献

以下是关于Phospho-RAF1(Y340)抗体的3篇参考文献，按文献发表时间由近及远排列：

---

1. **文献名称**: *"RAF1 phosphorylation at tyrosine 340/341 regulates its interaction with 14-3-3 proteins and cell migration"*

**作者**: Tran, T.H. et al.

**摘要**: 研究揭示了RAF1在Y340/Y341位点的磷酸化通过影响其与14-3-3蛋白的结合，调控细胞迁移和侵袭能力，并利用Phospho-RAF1(Y340)抗体验证了该位点在乳腺癌模型中的作用。

---

2. **文献名称**: *"Structural basis of autoinhibition and phosphorylation-dependent activation of RAF1"*

**作者**: Park, E. et al.

**摘要**: 通过结构生物学分析，阐明了RAF1激酶的自抑制机制及Y340磷酸化对抗体检测的构象变化影响，研究使用该抗体确认了磷酸化位点对RAF1活化的必要性。

---

3. **文献名称**: *"Targeting RAF1 kinase in KRAS-mutant cancers: Phosphorylation dynamics and therapeutic implications"*

**作者**: Chen, S.H. et al.

**摘要**: 在KRAS突变肿瘤模型中，研究通过Phospho-RAF1(Y340)抗体检测发现，RAF1的Y340磷酸化水平与MEK/ERK通路激活及靶向药物敏感性相关，为临床治疗提供新靶点。

---

**备注**：Y340磷酸化位点在不同物种或文献中可能标注为Y341.具体需结合实验上下文确认。以上文献为模拟示例，实际引用请以真实论文为准。

背景信息

The Phospho-RAF1 (Y340) antibody is a critical tool for studying the regulation of the RAF/MEK/ERK signaling pathway, which plays a central role in cellular proliferation, differentiation, and survival. RAF1 (also known as CRAF), a serine/threonine kinase, becomes activated through a multi-step process involving RAS-GTP binding, membrane recruitment, and phosphorylation. Phosphorylation at tyrosine 340 (Y340) is associated with RAF1 activation, though its exact role remains debated. Some studies suggest Y340 phosphorylation enhances RAF1 interaction with 14-3-3 proteins, stabilizing its active conformation, while others propose it may fine-tune kinase activity or mediate crosstalk with other signaling pathways. This antibody specifically detects RAF1 phosphorylated at Y340. enabling researchers to study activation dynamics in response to growth factors, cytokines, or oncogenic stimuli. It is widely used in applications like Western blotting, immunofluorescence, and immunohistochemistry to investigate RAF1 signaling in cancer, developmental biology, and drug resistance. Validation typically includes knockout controls or phosphatase treatment to confirm phosphorylation-dependent signals. Researchers should verify species reactivity (commonly human, mouse, rat) and optimize experimental conditions, as phosphorylation levels may be transient or context-dependent. Its use has contributed to understanding RAF1 regulation in diseases, particularly in cancers with RAS/RAF mutations, and in evaluating therapeutic inhibitors targeting this pathway.

客户数据及评论

折叠内容

大包装询价

×