| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, Trk-A, gp140trk, p140-TrkA, NTRK1, MTC, TRK, TRKA |
| Entrez GeneID | 4914 |
| WB Predicted band size | 87.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TrkA Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y791 of human TrkA. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-TrkA(Y791)抗体的3篇参考文献及其摘要内容：
1. **文献名称**：*"TrkA activation by nerve growth factor is critical for survival of neuroblastoma cells"*
**作者**：Lucia et al.
**摘要**：该研究利用Phospho-TrkA(Y791)抗体检测神经母细胞瘤中TrkA的激活状态，发现Y791磷酸化是NGF介导的细胞存活信号通路的关键环节，并提示其作为预后标志物的潜力。
2. **文献名称**：*"Development and validation of a phospho-specific antibody for detecting TrkA tyrosine 791 phosphorylation"*
**作者**：Kim et al.
**摘要**：本文报道了一种高特异性Phospho-TrkA(Y791)抗体的开发，通过肽段免疫和表位验证，证实其适用于Western blot和免疫荧光，可用于研究TrkA在神经元分化中的动态磷酸化调控。
3. **文献名称**：*"Phosphorylation of TrkA at Y791 modulates pain signaling in dorsal root ganglia neurons"*
**作者**：Zhang et al.
**摘要**：研究使用Phospho-TrkA(Y791)抗体分析小鼠背根神经节中TrkA的磷酸化水平，发现Y791位点的磷酸化通过增强MAPK通路活性参与慢性疼痛的分子机制。
(注：上述文献为模拟示例，实际引用需根据具体论文调整。)
Phospho-TrkA (Y791) antibody is a specialized tool used to detect the activated form of the tropomyosin receptor kinase A (TrkA), a key receptor tyrosine kinase involved in nerve growth factor (NGF) signaling. TrkA, encoded by the *NTRK1* gene, binds NGF to regulate neuronal survival, differentiation, and pain sensitivity. Upon ligand binding, TrkA undergoes dimerization and autophosphorylation at specific tyrosine residues, including Y791. This phosphorylation event serves as a critical docking site for adaptor proteins (e.g., SHC, PLC-γ), initiating downstream signaling cascades like MAPK/ERK, PI3K/AKT, and PKC pathways, which drive cellular responses.
The Y791 residue, located in the kinase domain, is associated with TrkA’s sustained activation and interaction with effector molecules. Phospho-TrkA (Y791) antibodies are designed to recognize this phosphorylation-specific epitope, enabling researchers to assess TrkA activation status in experimental models. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to study TrkA signaling in physiological contexts (e.g., neural development) or pathological conditions, including neuroblastoma, breast cancer, and chronic pain disorders. Dysregulated TrkA signaling, often linked to mutations or overexpression, is a therapeutic target; thus, monitoring Y791 phosphorylation helps evaluate drug efficacy (e.g., tyrosine kinase inhibitors). Validation of these antibodies typically involves phosphorylation-dependent specificity checks using cell lines with/without NGF stimulation or kinase-inactive mutants. Proper controls ensure accurate interpretation of TrkA activation dynamics in disease mechanisms or drug discovery.
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