| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, LEO1, RDL |
| Entrez GeneID | 123169 |
| WB Predicted band size | 75.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Leo1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S551 of human Leo1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-Leo1(S551)抗体的3篇参考文献示例(内容为虚构，仅供参考，实际文献需自行检索验证)：
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1. **文献名称**: *Phosphorylation of Leo1 at S551 Modulates RNA Polymerase II Transcription*
**作者**: Zhang Y, et al.
**摘要**: 本研究揭示了Leo1蛋白S551位点的磷酸化通过PAF复合体调控RNA聚合酶II的转录延伸过程，利用Phospho-Leo1(S551)抗体证实该修饰与基因启动子近端暂停释放相关。
2. **文献名称**: *CDK9-Dependent Phosphorylation of Leo1 Promotes Oncogenic Signaling in Breast Cancer*
**作者**: Lee H, et al.
**摘要**: 文章发现CDK9激酶介导的Leo1 S551磷酸化在乳腺癌中异常激活，通过Phospho-Leo1(S551)抗体检测到其表达水平与患者预后不良显著相关，提示其作为潜在治疗靶点。
3. **文献名称**: *Dynamic Phosphorylation of Leo1 Regulates Histone H2B Ubiquitination during Cell Differentiation*
**作者**: Smith J, et al.
**摘要**: 研究利用Phospho-Leo1(S551)特异性抗体，证明该修饰通过调控PAF复合体与RNF20/E3连接酶的相互作用，影响组蛋白H2B泛素化及干细胞多能性维持。
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**注意**：以上文献为示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献。若该位点研究较少，建议扩展关键词(如“Leo1 phosphorylation”或“PAF complex signaling”)寻找相关资源。
The Phospho-Leo1(S551) antibody is designed to detect the phosphorylation status of Leo1 (RNA Polymerase II-Associated Factor 1 Homolog) at serine residue 551. Leo1 is a core subunit of the PAF1 complex, which plays critical roles in transcriptional elongation, histone modification, and mRNA processing. Phosphorylation at S551 is implicated in regulating Leo1’s interaction with other components of the PAF1 complex or RNA polymerase II, potentially influencing chromatin remodeling and gene expression dynamics. This modification may also link Leo1 to cellular stress responses, cell cycle progression, or developmental pathways.
The antibody is commonly used in molecular biology research to study post-translational regulation of Leo1. particularly in contexts like cancer, stem cell differentiation, or epigenetic reprogramming. Specific applications include Western blotting, immunoprecipitation, or immunofluorescence to assess phosphorylation-dependent localization or activity. Validation typically involves testing in knockout models, phosphatase-treated samples, or phosphorylation-mimetic mutants.
Research utilizing this antibody could shed light on how Leo1 phosphorylation modulates transcriptional fidelity, coordinates with signaling pathways (e.g., MAPK or CDK cascades), or contributes to diseases like leukemia, where PAF1 complex dysregulation is observed. Always verify antibody specificity for phosphorylated S551 in experimental systems to avoid cross-reactivity.
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