| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Probable asparagine--tRNA ligase, mitochondrial, Asparaginyl-tRNA synthetase, AsnRS, NARS2 |
| Entrez GeneID | 79731 |
| WB Predicted band size | 54.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human NARS2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于NARS2抗体的3篇示例文献的概括(注：由于NARS2研究较新，以下内容为模拟文献，实际文献需通过PubMed或专业数据库检索确认)：
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1. **文献名称**: *NARS2 mutations cause autosomal recessive mitochondrial encephalomyopathy*
**作者**: Vanlander AV, et al.
**摘要**: 本研究首次报道了NARS2基因突变与线粒体脑肌病的关联，通过Western blot和免疫组化实验验证了患者组织中NARS2蛋白水平显著降低，使用特异性抗体证实了该酶在线粒体中的定位异常。
2. **文献名称**: *Functional characterization of NARS2 aspartyl-tRNA synthetase in mitochondrial translation*
**作者**: Diodato D, et al.
**摘要**: 该研究利用NARS2抗体探究了该酶在哺乳动物线粒体翻译中的作用，发现其缺陷导致tRNA天冬氨酸氨基酰化功能受损，抗体检测显示其与线粒体核糖体的相互作用在病理条件下被破坏。
3. **文献名称**: *Development of a monoclonal antibody against human NARS2 for diagnostic applications*
**作者**: Mizuguchi T, et al.
**摘要**: 研究团队开发了一种高特异性抗人NARS2单克隆抗体，验证了其在ELISA和免疫荧光中的灵敏度，为线粒体疾病的快速筛查提供了新工具。
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**建议**：如需真实文献，可通过PubMed搜索关键词“NARS2 antibody”或“NARS2 mitochondrial disease”，并筛选涉及抗体应用或疾病机制的研究。
NARS2 (mitochondrial asparaginyl-tRNA synthetase 2) is an enzyme encoded by the *NARS2* gene, belonging to the class II family of aminoacyl-tRNA synthetases (aaRSs). It catalyzes the attachment of asparagine to its cognate mitochondrial tRNA (tRNA\(^{Asn}\)), a critical step in mitochondrial protein synthesis. As a homodimeric enzyme, NARS2 requires ATP for its activity and functions within mitochondria to support the translation of mitochondrial DNA-encoded respiratory chain components. Mutations in *NARS2* are linked to mitochondrial disorders, including Alpers-like syndrome, progressive encephalopathy, epilepsy, sensorineural hearing loss, and Leigh syndrome, reflecting its essential role in energy metabolism and neuronal function.
NARS2 antibodies are tools used to study its expression, localization, and interactions in cellular models or patient tissues. They aid in diagnosing mitochondrial diseases, elucidating mechanisms of tRNA charging defects, and exploring genotype-phenotype correlations. Research using these antibodies has highlighted tissue-specific expression patterns, with high levels in metabolically active organs like the brain, liver, and skeletal muscle. Additionally, NARS2 antibodies contribute to investigating mitochondrial dysfunction in neurodegeneration and aging. As mitochondrial aaRSs gain attention as therapeutic targets, NARS2 antibodies also support drug development and biomarker discovery for related disorders.
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