| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KIAK0002 |
| WB Predicted band size | 33 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CCND2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CDK5R1(p35)抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *Cyclin-dependent kinase 5 in synaptic plasticity, learning and memory*
**作者**: Lai KO, Ip NY
**摘要**: 该研究探讨了CDK5/p35复合体在突触可塑性和记忆形成中的作用。作者通过免疫组化和Western blot分析，使用特异性p35抗体证明CDK5/p35在海马神经元中的动态分布，并揭示其通过磷酸化下游底物调控突触功能。
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2. **文献名称**: *p35 is required for CDK5 activation during photoreceptor degeneration in the retina*
**作者**: Sanada K, et al.
**摘要**: 研究利用p35特异性抗体检测视网膜变性模型中CDK5的激活机制。结果显示，p35在光感受器细胞中的表达上调促进了CDK5的活性，导致神经元凋亡，提示p35-CDK5通路在退行性疾病中的关键作用。
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3. **文献名称**: *Proteolytic cleavage of p35 to p25 dysregulates CDK5 activity in Alzheimer's disease*
**作者**: Patrick GN, et al.
**摘要**: 该文献通过Western blot和免疫沉淀技术，使用抗p35抗体证明阿尔茨海默病患者脑组织中p35异常裂解为p25的现象。这种裂解导致CDK5持续激活，进而引发tau蛋白过度磷酸化和神经纤维缠结形成。
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以上文献均涉及p35抗体的实验应用(如蛋白检测、定位分析)，并揭示了CDK5/p35在神经系统功能及疾病中的分子机制。
The CDK5R1 (p35) antibody is a crucial tool for studying the regulatory subunit of cyclin-dependent kinase 5 (CDK5), a serine/threonine kinase with diverse roles in neuronal development, synaptic function, and neurodegenerative diseases. The CDK5R1 gene encodes p35. a neuron-specific activator that binds CDK5 to form an active complex. Unlike classical cyclins, p35 stabilizes CDK5 and directs its substrate specificity, enabling its involvement in processes like neuronal migration, axon guidance, and dopamine signaling. Dysregulation of the CDK5/p35 complex is implicated in Alzheimer’s disease, Parkinson’s disease, and neurodevelopmental disorders, often linked to p35 cleavage into a hyperactive p25 fragment under pathological conditions.
The CDK5R1 (p35) antibody detects endogenous p35 protein, facilitating research into its expression, localization, and post-translational modifications. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore p35's role in healthy and diseased states. Researchers also employ this antibody to investigate CDK5 signaling cascades, synaptic plasticity, and mechanisms underlying neurodegeneration. Specificity validation (e.g., knockout controls) is critical due to p35's structural similarity to p25 and its low abundance in non-neuronal tissues. This antibody remains indispensable for unraveling CDK5/p35 biology and its therapeutic relevance in neurological disorders.
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