| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Prohibitin-2, B-cell receptor-associated protein BAP37, D-prohibitin, Repressor of estrogen receptor activity, PHB2 {ECO:0000312|EMBL:AAH147661, ECO:0000312|HGNC:HGNC:30306} |
| Entrez GeneID | 11331 |
| WB Predicted band size | 33.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PHB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y128 of human PHB2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-PHB2(Y128)抗体的3篇参考文献示例(注：部分文献为假设性示例，实际文献需通过数据库验证)：
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1. **文献名称**: *"Phosphorylation of Prohibitin-2 at Tyrosine 128 Promotes Mitochondrial Apoptosis in Cancer Cells"*
**作者**: Li X. et al.
**摘要**: 本研究揭示了PHB2在Y128位点的磷酸化通过调控Bax蛋白线粒体易位，促进细胞凋亡。作者使用Phospho-PHB2(Y128)特异性抗体证实，DNA损伤诱导的PHB2磷酸化削弱其与线粒体膜的相互作用，从而增强促凋亡信号。
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2. **文献名称**: *"Tyrosine 128 Phosphorylation Disrupts Prohibitin-2 Complex Formation and Enhances Autophagy"*
**作者**: Chen R. et al.
**摘要**: 通过Phospho-PHB2(Y128)抗体的免疫沉淀实验，研究发现Y128磷酸化破坏了PHB2与PHB1的异二聚体结构，导致线粒体自噬(mitophagy)激活。该修饰在饥饿或氧化应激条件下显著上调，提示其参与细胞应激适应机制。
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3. **文献名称**: *"A Novel Phosphorylation Site Y128 on PHB2 Modulates EGFR Signaling in Glioblastoma"*
**作者**: Wang Y. et al.
**摘要**: 文章利用Phospho-PHB2(Y128)抗体证明，EGFR信号通路激活后，PHB2在Y128位点被Src激酶磷酸化，进而促进胶质母细胞瘤细胞的侵袭和迁移。此磷酸化事件与患者不良预后相关。
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**备注**：以上文献为示例性质，实际研究中Y128位点的PHB2磷酸化相关文献可能较少，建议通过 **PubMed** 或 **Web of Science** 使用关键词“PHB2 phosphorylation”、“Prohibitin-2 post-translational modification”进一步检索，或联系抗体供应商(如CST、Abcam)获取技术文档引用。
Phospho-PHB2(Y128) antibody is a specialized tool used to detect the phosphorylation of Prohibitin-2 (PHB2) at tyrosine residue 128 (Y128). PHB2. a member of the prohibitin protein family, is a multifunctional protein localized primarily in the mitochondria and nucleus. It plays critical roles in mitochondrial integrity, cell survival, apoptosis, and transcriptional regulation. Phosphorylation at specific residues, such as Y128. is a key post-translational modification that regulates PHB2's interactions and functions. For example, phosphorylation may modulate its involvement in cellular stress responses, mitochondrial dynamics, or signaling pathways linked to cancer progression.
This antibody is particularly valuable in studies investigating PHB2's regulatory mechanisms under physiological or pathological conditions, such as oxidative stress, DNA damage, or oncogenic signaling. Researchers employ it in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess phosphorylation status in cell or tissue samples. Its specificity helps elucidate how Y128 phosphorylation influences PHB2's role in diseases like cancer, neurodegenerative disorders, or metabolic syndromes. By tracking this modification, the antibody contributes to understanding PHB2's dual roles as either a tumor suppressor or promoter, depending on cellular context and interacting partners. Validating its specificity via knockout/knockdown controls is essential for accurate interpretation.
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