| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Growth/differentiation factor 3, GDF-3, GDF3 |
| Entrez GeneID | 9573 |
| WB Predicted band size | 41.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GDF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-197 amino acids from the Central region of human GDF3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于GDF3抗体的3篇模拟参考文献(基于领域内常见研究方向，仅供参考)：
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1. **文献名称**：*GDF3 maintains pluripotency of embryonic stem cells by regulating Nodal signaling*
**作者**：Chen, C., Ware, S.M., & Shen, M.M.
**摘要**：本研究利用GDF3特异性抗体探究了GDF3在小鼠胚胎干细胞多能性维持中的作用。通过免疫沉淀和Western blot分析，发现GDF3通过调控Nodal/Smad2/3信号通路抑制分化，抗体特异性验证为关键实验依据。
2. **文献名称**：*Development and validation of a monoclonal antibody for specific detection of GDF3 in human tissues*
**作者**：Li, Y., Zhang, H., & Wang, J.
**摘要**：文章报道了一种高特异性抗人GDF3单克隆抗体的开发，并通过免疫组化、流式细胞术和敲低实验验证其可靠性。该抗体成功应用于乳腺癌和结肠癌组织中GDF3蛋白表达的临床样本分析。
3. **文献名称**：*GDF3 as a biomarker in early embryonic development: Insights from antibody-mediated functional studies*
**作者**：Doe, J., Smith, R., & Lee, K.
**摘要**：研究通过GDF3中和抗体阻断其功能，结合斑马鱼模型发现GDF3在胚胎中胚层形成中起关键作用。抗体介导的抑制实验表明GDF3缺失导致体轴发育异常，提示其潜在治疗靶点价值。
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**注**：以上文献为模拟示例，实际引用时请通过PubMed或Google Scholar检索真实文献并核对信息。
GDF3 (Growth Differentiation Factor 3), a member of the TGF-β (transforming growth factor-beta) superfamily, is a secreted signaling protein involved in embryonic development, particularly in eye formation, skeletal patterning, and stem cell differentiation. It shares structural homology with BMP (bone morphogenetic protein) ligands but exhibits distinct functional roles, often acting as a context-dependent regulator of BMP signaling. GDF3 is expressed during early embryogenesis, playing a role in mesoderm induction and cell fate specification. Its signaling pathway typically involves binding to Activin receptors or BMP receptors, with downstream effects mediated via SMAD proteins or non-canonical pathways.
Antibodies targeting GDF3 are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous GDF3 in tissues or cultured cells. These antibodies also facilitate research into GDF3's role in developmental disorders, such as Klippel-Feil syndrome, and its dual functionality in cancer—acting as a tumor suppressor in some contexts while promoting metastasis in others. Commercial GDF3 antibodies are often raised against specific epitopes, with validation including knockout controls to ensure specificity. Recent studies emphasize their utility in exploring GDF3's therapeutic potential in regenerative medicine, particularly in modulating stem cell pluripotency and tissue repair mechanisms.
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