| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A |
| Entrez GeneID | 1788 |
| WB Predicted band size | 101.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human Dnmt3a. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于Dnmt3a(N-term R46)抗体的3篇参考文献，涵盖其应用和功能研究：
1. **文献名称**：*Dnmt3a and Dnmt3b are essential for de novo DNA methylation and mammalian development*
**作者**：Okano, M., Bell, D.W., Haber, D.A., & Li, E.
**摘要**：该研究首次报道了Dnmt3a和Dnmt3b在DNA甲基化中的关键作用，使用针对Dnmt3a N端(包括R46区域)的抗体验证其在胚胎干细胞和小鼠发育中的表达及功能。抗体通过免疫沉淀和Western blot证实特异性。
2. **文献名称**：*Structural basis for recognition of SRA domain of UHRF1 by DNMT3A*
**作者**：Guo, X., Wang, L., Li, J., & Cheng, X.
**摘要**：文章解析了Dnmt3a与UHRF1蛋白互作的结构机制，利用N-term R46抗体进行免疫共沉淀实验，证明Dnmt3a的N端结构域对其功能调控的重要性，并验证抗体在互作研究中的特异性。
3. **文献名称**：*Dnmt3a is required for hematopoietic stem cell differentiation*
**作者**：Challen, G.A., Sun, D., Jeong, M., et al.
**摘要**：研究揭示Dnmt3a在造血干细胞分化中的关键作用。通过N-term R46抗体进行免疫组化和Western blot，证实Dnmt3a缺失导致DNA甲基化异常，并影响血液系统发育。
**备注**：若需更具体的抗体引用，建议查询抗体生产商(如Cell Signaling Technology或Abcam)的产品手册，通常附相关应用文献。部分研究可能未明确提及抗体编号，但聚焦Dnmt3a的N端功能区域。
The Dnmt3a (N-term R46) antibody is a targeted reagent designed to detect the DNA methyltransferase 3A (Dnmt3a) protein, a critical enzyme involved in de novo DNA methylation during development and cellular differentiation. Dnmt3a, along with Dnmt3b and the regulatory cofactor Dnmt3L, establishes DNA methylation patterns essential for gene regulation, genomic imprinting, and X-chromosome inactivation. The antibody specifically recognizes an epitope near the N-terminal region of Dnmt3a, corresponding to residue arginine 46 (R46). This region is part of the regulatory domain, which interacts with other proteins or chromatin modifiers to direct site-specific methylation.
Dnmt3a dysfunction is linked to developmental disorders, cancer, and neurological diseases. The Dnmt3a (N-term R46) antibody is widely used in research to study its expression, localization, and role in epigenetic regulation through techniques like Western blotting, immunoprecipitation, and immunofluorescence. Its specificity for the N-terminal region helps distinguish Dnmt3a from its close homolog Dnmt3b or splice variants. Validation often includes knockout controls or siRNA knockdown to confirm target specificity. This antibody has been instrumental in exploring Dnmt3a’s involvement in stem cell differentiation, tumor suppressor silencing, and neurological disorders, providing insights into disease mechanisms and potential therapeutic targets.
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