| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MEK5, MKK5, PRKMK5 |
| Entrez GeneID | 5607 |
| WB Predicted band size | 50.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MAP2K5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from human MAP2K5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MAP2K5(S149)抗体的参考文献示例(内容为模拟概括，仅供参考)：
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1. **文献名称**：*"Phosphorylation of MAP2K5 at Serine 149 Modulates Cellular Stress Responses"*
**作者**：Chen L, et al.
**摘要**：本研究利用特异性MAP2K5(S149)抗体，揭示了该位点的磷酸化在氧化应激条件下对ERK5信号通路的激活作用，表明其在细胞凋亡和存活中的调控功能。
2. **文献名称**：*"Development and Validation of a Phospho-Specific Antibody for MEK5/ERK5 Signaling Studies"*
**作者**：Roberts JM, et al.
**摘要**：作者报道了一种针对MAP2K5 Ser149磷酸化位点的单克隆抗体的开发，验证了其在Western blot和免疫荧光中的特异性，并应用于检测心肌细胞中MEK5/ERK5通路的动态激活。
3. **文献名称**：*"MAP2K5 S149 Phosphorylation in Cancer Metastasis: Insights from Antibody-Based Profiling"*
**作者**：Wang H, et al.
**摘要**：通过MAP2K5(S149)抗体检测多种肿瘤样本，发现该位点的磷酸化水平与乳腺癌转移正相关，提示其作为潜在治疗靶点的价值。
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**注意**：以上文献为示例性内容，实际研究中请通过PubMed或Google Scholar等平台检索具体文献。若需真实文献，建议使用关键词“MAP2K5 Ser149 phosphorylation antibody”进行查询。
The MAP2K5 (S149) antibody is a specialized tool used to detect the phosphorylation of mitogen-activated protein kinase kinase 5 (MAP2K5. also known as MEK5) at serine 149. MAP2K5 is a key component of the MAPK signaling cascade, specifically within the MEK5/ERK5 pathway, which regulates cellular responses such as proliferation, differentiation, and survival under stress. Phosphorylation at S149 is critical for MAP2K5 activation, enabling it to phosphorylate and activate its downstream target, extracellular signal-regulated kinase 5 (ERK5). This pathway is implicated in cardiovascular development, neuronal survival, and cancer progression.
The MAP2K5 (S149) antibody is designed to recognize the activated form of MAP2K5. making it valuable for studying ERK5 pathway dynamics in physiological and pathological contexts. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess MAP2K5 activation status in cell lines, tissues, or disease models. Its specificity for the phosphorylated S149 residue ensures accurate detection of pathway activity, aiding investigations into mechanisms underlying cancer, inflammation, or cardiovascular diseases. Proper validation, including knockout controls or peptide competition assays, is essential to confirm antibody specificity.
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