| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cytosol aminopeptidase, Leucine aminopeptidase 3, LAP-3, Leucyl aminopeptidase, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase, LAP3, LAPEP, PEPS |
| Entrez GeneID | 51056 |
| WB Predicted band size | 56.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the N-terminal region of human LAP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于LAP3(N-term)抗体的模拟参考文献示例(仅供参考，非真实文献)：
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1. **文献名称**：*Characterization of LAP3 N-terminal antibody in human cancer cell lines*
**作者**：Smith A, et al.
**摘要**：本研究验证了一种新型LAP3(N-term)抗体的特异性，通过Western blot和免疫荧光技术证实其在多种癌细胞系中可特异性识别LAP3蛋白的N端结构域。研究还发现LAP3在结肠癌细胞中高表达，并与细胞增殖相关。
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2. **文献名称**：*Role of LAP3 in neuronal protein degradation and antibody-based detection*
**作者**：Chen L, et al.
**摘要**：文章利用LAP3(N-term)抗体探究了LAP3蛋白在神经元中的分布及其在错误折叠蛋白清除中的作用。实验表明，LAP3的N端区域对底物识别至关重要，抗体在小鼠脑组织切片中成功标记了LAP3的活性位点。
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3. **文献名称**：*Development and validation of a polyclonal antibody targeting the N-terminal domain of LAP3*
**作者**：Park J, et al.
**摘要**：该研究报道了一种兔源多克隆抗体的开发，针对LAP3蛋白的N端肽段(氨基酸1-50)。通过ELISA和免疫组化验证，抗体在肝组织样本中表现出高灵敏度和低交叉反应性，为LAP3的酶活性调控研究提供了工具。
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4. **文献名称**：*LAP3 as a biomarker in metabolic disorders: Insights from antibody-based assays*
**作者**：Gomez R, et al.
**摘要**：研究使用LAP3(N-term)抗体分析代谢疾病患者血清样本，发现LAP3水平与胰岛素抵抗显著相关。抗体的特异性通过siRNA敲低实验确认，支持其在临床诊断中的应用潜力。
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**备注**：以上内容为示例性模拟文献，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。
The LAP3 (N-term) antibody is designed to target the N-terminal region of leucine aminopeptidase 3 (LAP3), a member of the M1 metallopeptidase family. LAP3. also known as cystinyl aminopeptidase or puromycin-sensitive aminopeptidase, is a zinc-dependent enzyme involved in protein turnover, peptide processing, and cellular homeostasis. It plays roles in diverse biological processes, including antigen processing for MHC class I presentation, cell cycle regulation, and neuropeptide metabolism. Dysregulation of LAP3 has been implicated in cancer progression, neurodegenerative disorders, and reproductive health.
The LAP3 (N-term) antibody is commonly generated in rabbit or mouse hosts using synthetic peptides or recombinant protein fragments corresponding to the N-terminal domain of human LAP3. This region is relatively conserved across species, allowing cross-reactivity in models like mouse and rat. Validated for applications such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF), the antibody helps detect endogenous LAP3 expression levels and localization in tissues or cultured cells. Researchers utilize it to study LAP3's involvement in proteasomal degradation pathways, its role in cell proliferation/apoptosis, and its potential as a biomarker in diseases like glioblastoma or ovarian cancer. Specificity is often confirmed using knockout controls or siRNA-mediated silencing. Its development supports ongoing exploration of LAP3's enzymatic mechanisms and therapeutic targeting in pathological conditions.
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