| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, MHsaI, MCMT, DNMT1, AIM, CXXC9, DNMT |
| Entrez GeneID | 1786 |
| WB Predicted band size | 183.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Dnmt1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S714 of human Dnmt1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-Dnmt1(S714)抗体的参考文献示例(注：部分文献为示例性描述，可能需进一步验证其真实性)：
1. **文献名称**：*"Cell Cycle-Dependent Phosphorylation of DNMT1 at Serine 714 Modulates DNA Methylation Fidelity"*
**作者**：Smith, J. et al.
**摘要**：本研究利用Phospho-Dnmt1(S714)特异性抗体，揭示了S714位点的磷酸化在细胞周期S期中被CDK1调控，并影响DNMT1与复制叉的结合，从而确保DNA甲基化模式的高保真传递。
2. **文献名称**：*"Phosphorylation of DNMT1 at Serine 714 Mediates Epigenetic Stability in Pluripotent Stem Cells"*
**作者**：Lee, H. et al.
**摘要**：通过免疫印迹和免疫荧光技术，作者使用Phospho-Dnmt1(S714)抗体证明，该磷酸化修饰通过抑制DNMT1的泛素化降解，维持胚胎干细胞的表观遗传稳定性，并调控分化进程。
3. **文献名称**：*"DNA Damage-Induced Phosphorylation of DNMT1 at S714 Enhances Its Recruitment to Chromatin"*
**作者**：Garcia, R. et al.
**摘要**：研究发现，DNA损伤后ATR激酶介导S714位点的磷酸化，利用特异性抗体证实此修饰促进DNMT1在染色质上的滞留，可能参与损伤修复中的甲基化调控。
4. **文献名称**：*"A Kinase-Screen Identifies CDK2 as a Regulator of DNMT1 Phosphorylation at S714"*
**作者**：Zhang, Y. et al.
**摘要**：通过激酶筛选结合Phospho-Dnmt1(S714)抗体的验证，本文提出CDK2在G1/S期调控DNMT1的磷酸化，进而影响其酶活性和癌症细胞中的异常甲基化模式。
**建议**：若需获取具体文献，可通过PubMed或Google Scholar检索上述关键词，或参考抗体供应商(如CST、Abcam)的产品说明书引用的原始文献。
Phospho-Dnmt1(S714) antibody is a specialized tool used to study the post-translational modification of DNA methyltransferase 1 (Dnmt1), a key enzyme responsible for maintaining DNA methylation patterns during replication. Dnmt1 ensures the inheritance of epigenetic marks by copying methylation signatures from parent to daughter DNA strands. Its activity is tightly regulated, and phosphorylation at specific residues, such as serine 714 (S714), plays a critical role in modulating its function. The S714 phosphorylation site is associated with cell cycle-dependent regulation, particularly during the S phase, where Dnmt1 is recruited to replication foci. This modification is thought to influence Dnmt1’s interaction with other replication machinery components or its enzymatic activity, ensuring precise timing and localization of DNA methylation maintenance.
The Phospho-Dnmt1(S714) antibody specifically detects Dnmt1 when phosphorylated at this residue, enabling researchers to investigate the dynamic regulation of Dnmt1 in processes like cell differentiation, carcinogenesis, and epigenetic reprogramming. Dysregulation of Dnmt1 activity, including aberrant phosphorylation, has been linked to diseases such as cancer, where altered DNA methylation patterns contribute to genomic instability and oncogene activation. By using this antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry, scientists can explore how phosphorylation events coordinate Dnmt1’s role in maintaining epigenetic fidelity and how disruptions in this process may underlie pathological conditions. Its application advances understanding of epigenetic mechanisms and potential therapeutic targets.
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