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Rabbit Polyclonal Phospho-DNA-PK(T2609) Antibody

  • 中文名： Phospho-DNA-PK(T2609)抗体
  • 别    名： DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNPK1, p460, PRKDC, HYRC, HYRC1
货号: IPDX30238
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesDNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNPK1, p460, PRKDC, HYRC, HYRC1
Entrez GeneID5591
WB Predicted band size469.1kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis DNA-PK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T2609 of human DNA-PK.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于Phospho-DNA-PK(T2609)抗体的假设性参考文献示例，基于典型研究场景整理而成：

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1. **文献名称**: *"DNA-PK autophosphorylation at Thr2609 is essential for DNA double-strand break repair"*

**作者**: Chen BP, et al.

**摘要**: 本研究利用Phospho-DNA-PK(T2609)特异性抗体，证实DNA损伤后Thr2609位点的自磷酸化对DNA-PK激酶活性激活至关重要，并促进非同源末端连接(NHEJ)修复通路。

2. **文献名称**: *"A phospho-specific antibody reveals differential DNA-PK activation in response to ionizing radiation and cisplatin"*

**作者**: Dobbs TA, et al.

**摘要**: 通过开发并验证Phospho-DNA-PK(T2609)抗体，作者发现电离辐射显著诱导该位点磷酸化，而顺铂处理则无此效应，提示DNA损伤类型特异性激活机制。

3. **文献名称**: *"Validation of a DNA-PK Thr2609 phospho-specific antibody for functional studies in cancer models"*

**作者**: Williams RS, et al.

**摘要**: 文献详细描述了该抗体的特异性验证(如敲除/突变实验)，并应用于临床肿瘤样本，证明DNA-PK T2609磷酸化水平与放疗敏感性相关。

4. **文献名称**: *"Phosphorylation dynamics of DNA-PK at Thr2609 regulate its interaction with Artemis during V(D)J recombination"*

**作者**: Meek K, et al.

**摘要**: 利用该抗体进行免疫沉淀实验，揭示T2609磷酸化调控DNA-PK与Artemis的结合，影响淋巴细胞发育中的V(D)J重组效率。

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**注**：以上文献为示例性质，实际引用时需以真实发表的论文为准。建议通过PubMed或Google Scholar以关键词“DNA-PK Thr2609 phosphorylation antibody”进一步检索。

背景信息

The Phospho-DNA-PK(T2609) antibody is a specialized tool used to detect the phosphorylation status of DNA-dependent protein kinase (DNA-PK) at threonine 2609. a critical post-translational modification linked to its activation and function. DNA-PK is a serine/threonine kinase composed of a catalytic subunit (DNA-PKcs) and the Ku70/Ku80 regulatory complex. It plays a central role in the non-homologous end joining (NHEJ) pathway, the primary mechanism for repairing DNA double-strand breaks (DSBs) in mammals. Phosphorylation at T2609. located in the putative activation loop of DNA-PKcs, is induced by DNA damage and is associated with kinase activation, autophosphorylation, and recruitment of repair proteins. This modification serves as a biomarker for monitoring DNA-PK activity in response to genotoxic stressors, such as ionizing radiation or chemotherapeutic agents. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study DSB repair dynamics, genomic instability, and therapeutic responses in cancer models. Its specificity for the phosphorylated form makes it valuable for elucidating DNA damage signaling pathways and evaluating inhibitors targeting DNA-PK in preclinical studies. Dysregulation of DNA-PK activity is implicated in cancer progression, radiation resistance, and immune disorders, underscoring the antibody's relevance in both basic research and drug development.

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