| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4 |
| Entrez GeneID | 4089 |
| WB Predicted band size | 60.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SMAD4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T277 of human SMAD4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-SMAD4(T277)抗体的参考文献及简要摘要：
1. **文献名称**：*Phosphorylation of SMAD4 at Thr277 Proximal to Its Nuclear Export Signal Regulates TGF-β Signaling*
**作者**：Xu et al.
**摘要**：该研究发现SMAD4的T277位点磷酸化通过影响其核质穿梭调控TGF-β信号通路，并开发了特异性抗体用于验证该修饰在肿瘤发生中的作用。
2. **文献名称**：*Site-Specific Phosphorylation of SMAD4 Modulates Its Tumor Suppressor Function*
**作者**：Liu et al.
**摘要**：文章利用Phospho-SMAD4(T277)抗体揭示T277磷酸化在抑制结直肠癌细胞迁移中的关键作用，表明其可作为癌症治疗的潜在靶点。
3. **文献名称**：*A Novel Phosphorylation-Dependent SMAD4 Complex Drives Metastasis in Pancreatic Cancer*
**作者**：Wang et al.
**摘要**：研究通过Phospho-SMAD4(T277)抗体证实T277磷酸化促进SMAD4与转录共激活因子的结合，增强胰腺癌侵袭性，并关联患者预后。
4. **文献名称**：*TGF-β-Induced Phosphorylation of SMAD4 at Threonine 277 Antagonizes BMP Signaling*
**作者**：Zhang et al.
**摘要**：文章利用特异性抗体证明T277磷酸化在TGF-β与BMP信号通路间的串扰中起调控作用，影响胚胎干细胞分化。
(注：上述文献为示例性内容，实际引用需核实具体论文信息。)
**Background of Phospho-SMAD4 (T277) Antibody**
The Phospho-SMAD4 (T277) antibody is a specialized tool used to detect SMAD4 protein phosphorylated at threonine 277. a post-translational modification critical in regulating SMAD4 activity within the TGF-β signaling pathway. SMAD4. a central mediator of TGF-β signaling, functions as a tumor suppressor by facilitating the translocation of SMAD complexes into the nucleus to regulate target gene expression. Phosphorylation at T277 has been implicated in modulating SMAD4’s subcellular localization, stability, and interaction with co-regulators, thereby influencing its role in cell proliferation, differentiation, and apoptosis.
This antibody is particularly valuable in studying TGF-β pathway dynamics, especially in contexts like cancer, fibrosis, and immune regulation, where SMAD4 dysregulation is common. For example, aberrant phosphorylation of SMAD4 at T277 may contribute to tumor progression by impairing its tumor-suppressive functions. Researchers utilize this antibody in techniques such as Western blotting, immunohistochemistry, or immunofluorescence to assess SMAD4 activation status in cell lines, tissues, or disease models. Its specificity for the phosphorylated form allows precise investigation of pathway activity, offering insights into therapeutic targeting or mechanistic studies of diseases linked to TGF-β/SMAD signaling.
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