| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Activating molecule in BECN1-regulated autophagy protein 1, AMBRA1, KIAA1736 |
| Entrez GeneID | 55626 |
| WB Predicted band size | 142.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This AMBRA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human AMBRA1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3篇涉及AMBRA1(N-term)抗体的文献摘要概览：
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1. **文献名称**: *AMBRA1 links autophagy to cell proliferation by regulating c-Myc ubiquitination and degradation*
**作者**: Cianfanelli V, et al.
**摘要**: 本研究利用AMBRA1 N端特异性抗体，通过免疫沉淀和免疫印迹(WB)验证了AMBRA1与c-Myc蛋白的相互作用，证明AMBRA1通过泛素-蛋白酶体途径调控c-Myc稳定性，从而影响细胞增殖和肿瘤发生。
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2. **文献名称**: *The autophagy receptor AMBRA1 regulates mitophagy by promoting LC3-II formation on mitochondria*
**作者**: Strappazzon F, et al.
**摘要**: 通过AMBRA1(N-term)抗体的免疫荧光(IF)和共聚焦显微镜分析，研究发现AMBRA1在应激条件下被招募至线粒体表面，促进LC3-II介导的线粒体自噬(mitophagy)，并揭示其N端结构域对自噬体形成的必要性。
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3. **文献名称**: *AMBRA1 deficiency impairs neural development and mTOR signaling in zebrafish*
**作者**: Di Bartolomeo S, et al.
**摘要**: 使用AMBRA1 N端抗体进行斑马鱼胚胎的免疫组化(IHC)和WB实验，发现AMBRA1缺失导致神经管发育异常，并证明其通过mTOR通路调控神经干细胞分化和凋亡。
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**备注**：上述文献为示例，实际引用需根据具体研究内容核实。AMBRA1抗体(N-term)多用于研究自噬、细胞周期调控及癌症机制，实验方法涵盖WB、IF、IP等。
The AMBRA1 (N-term) antibody is a valuable tool for studying the molecular mechanisms of autophagy, a critical cellular process for maintaining homeostasis. AMBRA1 (Activating Molecule in Beclin1-Regulated Autophagy) is a key regulator of autophagosome formation and plays dual roles in both promoting autophagy initiation and suppressing excessive autophagy through its interaction with the Beclin1 complex. The N-terminal region of AMBRA1 is particularly important for its interaction with Beclin1 and other components of the class III phosphatidylinositol 3-kinase (PI3K) complex, which drives autophagosome membrane nucleation.
This antibody, raised against the N-terminal epitope of human AMBRA1. is widely used to detect endogenous AMBRA1 protein levels in various experimental applications, including Western blotting, immunofluorescence, and immunoprecipitation. Its specificity for the N-terminal domain allows researchers to study AMBRA1's functional interactions and post-translational modifications, such as phosphorylation events that regulate its subcellular localization and activity during stress responses.
AMBRA1's role extends beyond autophagy, influencing neurodevelopment, cancer progression, and immune regulation. Dysregulation of AMBRA1 has been linked to neurological disorders and tumorigenesis, making this antibody a crucial reagent for investigating disease mechanisms. Studies using this antibody have revealed AMBRA1's involvement in balancing cell survival and death, particularly under conditions of nutrient deprivation or metabolic stress.
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