| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand, Fused in glioblastoma, PDZ protein interacting specifically with TC10, PIST, GOPC, CAL, FIG |
| Entrez GeneID | 57120 |
| WB Predicted band size | 50.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PIST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the Central region of human PIST. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PIST(GOPC)抗体的3篇模拟文献摘要，涵盖不同研究方向：
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1. **文献名称**：*The Role of PIST in Synaptic Protein Organization*
**作者**：Chen L, et al.
**摘要**：本研究利用PIST特异性抗体，通过免疫共沉淀和免疫荧光技术，揭示了PIST蛋白在神经元突触后膜蛋白(如GABA_B受体)运输中的调控作用，表明其缺陷可能导致突触功能障碍。
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2. **文献名称**：*Overexpression of GOPC in Breast Cancer and Its Clinical Implications*
**作者**：Wang Y, et al.
**摘要**：通过Western blot和免疫组化分析，研究发现GOPC/PIST在乳腺癌组织中高表达，且与肿瘤转移相关。使用商业化PIST抗体证实其作为潜在生物标志物的可能性。
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3. **文献名称**：*Development of a High-Affinity Monoclonal Antibody Against Human PIST Protein*
**作者**：Kimura T, et al.
**摘要**：本文报道了一种新型抗PIST单克隆抗体的开发，通过质谱和免疫印迹验证其特异性。该抗体成功应用于细胞系中PIST与CFTR蛋白相互作用的共定位研究。
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**备注**：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science以“GOPC antibody”或“PIST protein detection”为关键词检索最新论文。
PIST (PDZ domain protein interacting specifically with TC10), also known as GOPC (Golgi-associated PDZ and coiled-coil motif-containing protein), is a Golgi-resident protein involved in intracellular trafficking and protein sorting. It contains an N-terminal coiled-coil domain and a C-terminal PDZ domain, enabling interactions with various membrane proteins, including receptors, channels, and transporters. PIST/GOPC regulates vesicle transport, synaptic formation, and membrane protein localization by acting as a scaffolding molecule. Its association with TC10. a Rho GTPase, links it to cytoskeletal reorganization and cell polarity.
PIST has been implicated in diverse physiological processes, such as spermatogenesis, neurotransmitter release, and epithelial cell polarization. Dysregulation of PIST is associated with cancers, neurological disorders, and ciliopathies. For instance, aberrant PIST expression may disrupt protein trafficking, contributing to tumor progression or neurodegenerative conditions like Alzheimer’s disease.
PIST antibodies are essential tools for studying its expression, localization, and interactions in cellular models. They enable detection via Western blotting, immunofluorescence, and immunoprecipitation, aiding research into PIST’s role in disease mechanisms and potential therapeutic targets. These antibodies are validated for specificity to ensure accurate identification of PIST isoforms and interaction partners.
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