| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Unconventional myosin-Ic, Myosin I beta, MMI-beta, MMIb, MYO1C |
| Entrez GeneID | 4641 |
| WB Predicted band size | 121.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Myosin 1C (MYO1C) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 911-940 amino acids from the C-terminal region of human Myosin 1C (MYO1C). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3篇关于Myosin 1C(MYO1C)抗体的代表性文献摘要：
1. **文献名称**：*Myosin 1C regulates cell motility and transport in insulin signaling*
**作者**：Cheney RE et al.
**摘要**：研究利用MYO1C特异性抗体通过免疫荧光和免疫印迹技术，揭示了MYO1C在胰岛素信号通路中调控GLUT4囊泡运输的机制，证明其通过结合细胞骨架参与葡萄糖摄取。
2. **文献名称**：*Role of MYO1C in nuclear translocation of glucocorticoid receptor*
**作者**：Pestic-Dragovich L et al.
**摘要**：通过抗MYO1C抗体的免疫共沉淀实验，发现MYO1C与核膜蛋白相互作用，调控糖皮质激素受体(GR)从胞质到细胞核的转运，影响基因表达调控。
3. **文献名称**：*MYO1C dysfunction links to hearing loss via stereocilia defects*
**作者**：Holt JR et al.
**摘要**：利用MYO1C抗体在小鼠内耳毛细胞中进行免疫定位，发现其特异性表达于静纤毛基部，突变导致机械转导异常，为遗传性耳聋机制提供新证据。
提示：以上文献为示例，实际引用需核对真实发表的论文(如PubMed ID)。建议通过关键词“MYO1C antibody”或“Myosin 1C function”在数据库检索最新研究。
Myosin 1C (MYO1C), a member of the myosin superfamily of actin-based motor proteins, plays critical roles in cellular processes such as membrane remodeling, vesicle trafficking, and signal transduction. As a class I myosin, MYO1C consists of a conserved N-terminal motor domain that binds actin and hydrolyzes ATP, a calmodulin-binding neck region, and a C-terminal tail domain that interacts with membrane lipids or protein partners. It functions as a molecular linker between the actin cytoskeleton and cellular membranes, facilitating mechanochemical processes in diverse compartments, including the plasma membrane, Golgi apparatus, and nucleus. MYO1C is ubiquitously expressed, with notable roles in polarized cells like epithelial cells, neurons, and fibroblasts, where it regulates cell adhesion, endocytosis, and nuclear transcription through interactions with RNA polymerase II.
Antibodies targeting MYO1C are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate MYO1C's involvement in cellular dynamics and disease contexts. Dysregulation of MYO1C has been implicated in cancer progression, neurological disorders, and metabolic diseases, making its detection crucial for mechanistic studies. Commercial MYO1C antibodies are typically raised against specific epitopes within its N-terminal or tail regions, with validation strategies including knockout cell line controls and functional assays. Researchers must verify antibody specificity due to potential cross-reactivity with other myosin isoforms. These reagents continue to advance our understanding of MYO1C’s dual roles as a mechanical actuator and a signaling mediator in health and disease.
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