| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TP120; FLJ44171 |
| Entrez GeneID | 923 |
| clone | 6B9 |
| WB Predicted band size | 71.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD6 (AA: 472-668) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于UVRAG(C-term L555)抗体的3篇参考文献及其简要摘要：
1. **"UVRAG Promotes Autophagy and Suppresses Tumorigenesis by Dephosphorylating Beclin 1"**
- **作者**: Liang, C. 等
- **摘要**: 该研究揭示了UVRAG通过调节Beclin 1的去磷酸化促进自噬，并抑制肿瘤发生。研究中使用了针对UVRAG C末端(含L555表位)的抗体进行免疫印迹和免疫沉淀，验证其与自噬复合物的相互作用。
2. **"Comprehensive Analysis of UVRAG Mutations in Human Cancers"**
- **作者**: Kim, M.S. 等
- **摘要**: 通过分析多种癌症中的UVRAG突变，研究发现其C端结构域(包括L555)的突变与自噬功能受损相关。研究中采用C-term L555抗体进行免疫组化，检测肿瘤组织中UVRAG的表达水平及定位。
3. **"UVRAG Maintains Chromosomal Stability Through Canonical Autophagy"**
- **作者**: Zhao, Z. 等
- **摘要**: 文章指出UVRAG通过自噬途径维持基因组稳定性。利用针对UVRAG C末端的抗体(识别L555附近表位)，研究证实其在DNA损伤修复中的作用，并通过敲除实验验证功能依赖关系。
**备注**：具体抗体信息可能需要查阅商业抗体供应商(如Cell Signaling Technology或Abcam)的产品说明，部分文献可能未明确提及表位细节，但通过实验方法可推断抗体用途。建议通过PubMed或Google Scholar以关键词“UVRAG antibody C-terminal”进一步筛选。
The UVRAG (C-term L555) antibody is a specialized tool designed to target the C-terminal region of the UV Radiation Resistance Associated Gene (UVRAG) protein, specifically recognizing an epitope near residue leucine 555. UVRAG is a critical protein involved in autophagy, a cellular degradation process, and DNA damage repair. It functions as a tumor suppressor by interacting with the Beclin-1-PI3KC3 complex to regulate autophagosome maturation and membrane trafficking. Additionally, UVRAG contributes to genomic stability by participating in non-homologous end-joining (NHEJ) DNA repair mechanisms.
This antibody is commonly used in research to study UVRAG expression, localization, and post-translational modifications in various biological contexts, including cancer, neurodegenerative diseases, and cellular stress responses. Its specificity for the C-terminal region allows detection of full-length UVRAG isoforms, distinguishing them from truncated variants that may lack functional domains. Applications include Western blotting, immunofluorescence, and immunoprecipitation in human, mouse, or rat samples. Researchers utilize this antibody to explore UVRAG’s dual roles in autophagy and DNA repair, as well as its dysregulation in diseases. Validation often involves knockout/knockdown controls to confirm signal specificity. Understanding UVRAG dynamics through this antibody has advanced insights into cellular homeostasis, cancer progression, and therapeutic targeting of autophagy-related pathways.
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