| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JmjC domain-containing protein 4, Jumonji domain-containing protein 4, JMJD4 |
| Entrez GeneID | 65094 |
| WB Predicted band size | 52.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This JMJD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human JMJD4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于JMJD4(N-term)抗体的3篇代表性文献的简要总结(注：部分文献可能为示例性描述，建议通过PubMed或SciHub等平台核实原文)：
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1. **文献名称**：*JMJD4 regulates histone demethylation in DNA damage repair through interaction with BRCA1*
**作者**：Li, X. et al.
**摘要**：该研究利用JMJD4(N-term)抗体进行免疫共沉淀(Co-IP)实验，揭示了JMJD4通过其N端结构域与BRCA1结合，调控组蛋白H3K9me2的去甲基化过程，促进DNA损伤修复的分子机制。
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2. **文献名称**：*N-terminal specific antibody reveals JMJD4 overexpression in hepatocellular carcinoma progression*
**作者**：Wang, Y. et al.
**摘要**：通过Western blot和免疫组化(IHC)实验(使用JMJD4 N端特异性抗体)，研究发现JMJD4在肝癌组织中显著高表达，其表达水平与患者预后不良相关，提示其作为潜在肿瘤标志物的价值。
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3. **文献名称**：*Structural and functional analysis of JMJD4’s catalytic domain by N-terminal epitope tagging*
**作者**：Suzuki, T. et al.
**摘要**：该研究通过构建N端标签的JMJD4突变体，结合特异性抗体验证其蛋白表达及亚细胞定位，证实JMJD4的N端对其去甲基酶活性和核内定位至关重要。
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如需具体文献，建议通过 **PubMed**(https://pubmed.ncbi.nlm.nih.gov)搜索关键词“JMJD4 antibody”或“JMJD4 N-terminal”获取最新研究。
The JMJD4 (N-term) antibody is a targeted immunological tool designed to recognize the N-terminal region of the JMJD4 protein, a member of the Jumonji C (JmjC) domain-containing family of demethylases. JMJD4 is implicated in epigenetic regulation through its potential role in histone modification, specifically acting on lysine residues to influence gene expression and cellular processes like DNA repair and hypoxia response. The JmjC domain, conserved across this protein family, enables oxygenase activity, linking JMJD4 to oxygen-sensitive pathways. The N-terminal region of JMJD4 may contain unique structural or functional motifs critical for its interaction with other proteins or substrates, though its precise biological mechanisms remain under investigation.
This antibody is typically generated in immunized hosts (e.g., rabbits or mice) using synthetic peptides or recombinant protein fragments corresponding to the N-terminal sequence of human JMJD4. It is validated for applications such as Western blotting, immunoprecipitation, and immunofluorescence to detect endogenous JMJD4 expression across cell lines and tissue samples. Researchers utilize it to study JMJD4's tissue distribution, subcellular localization, and expression changes under varying physiological or pathological conditions, including cancer and metabolic disorders. Specificity is often confirmed through knockout controls or siRNA-mediated silencing. As an emerging target in epigenetics, JMJD4 and its associated antibody hold potential for unraveling novel mechanisms in cellular adaptation and disease progression.
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