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Rabbit Polyclonal Phospho-EP300(S89) Antibody

  • 中文名: Phospho-EP300(S89)抗体
  • 别    名: Histone acetyltransferase p300, p300 HAT, E1A-associated protein p300, EP300, P300
货号: IPDX30175
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesHistone acetyltransferase p300, p300 HAT, E1A-associated protein p300, EP300, P300
Entrez GeneID2033
WB Predicted band size264.2kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis EP300 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S89 of human EP300.
FormulationPurified antibody in TBS with 0.05% sodium azide.

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参考文献

以下是关于Phospho-EP300(S89)抗体的假设性参考文献示例(仅供参考,具体文献需根据实际研究查询):

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1. **文献名称**:*Phosphorylation of EP300 at Serine 89 Mediates DNA Damage Response*

**作者**:Smith J, et al.

**摘要**:本研究揭示了EP300在DNA损伤后被ATM激酶磷酸化(S89位点),促进其与BRCA1复合物的结合,从而增强同源重组修复能力,维持基因组稳定性。

2. **文献名称**:*Oncogenic Role of p-EP300(S89) in Hepatocellular Carcinoma*

**作者**:Zhang L, et al.

**摘要**:通过免疫组化分析发现,Phospho-EP300(S89)在肝癌组织中高表达,其磷酸化水平与患者预后不良相关,可能通过激活Wnt/β-catenin通路促进肿瘤进展。

3. **文献名称**:*AMPK-Dependent Phosphorylation of EP300 at S89 Regulates Metabolic Adaptation*

**作者**:Lee H, et al.

**摘要**:研究发现AMPK在能量应激下磷酸化EP300(S89),抑制其组蛋白乙酰转移酶活性,从而调控糖异生相关基因表达,影响细胞代谢重编程。

4. **文献名称**:*Dynamic Phosphorylation of EP300 at Ser89 Modulates Cell Cycle Progression*

**作者**:Johnson R, et al.

**摘要**:S89位点的磷酸化在G1/S期转换中显著上调,通过调控p21和Cyclin D1的转录影响细胞周期进程,提示其在癌症治疗中的潜在靶点价值。

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**注意**:以上为基于EP300功能研究的假设性文献,实际研究中需通过数据库(如PubMed)以关键词“Phospho-EP300 Ser89”或“EP300 S89 phosphorylation”查询具体文献。

背景信息

Phospho-EP300(S89) antibody specifically detects the phosphorylation of EP300 (E1A-binding protein p300) at serine 89. a post-translational modification critical for regulating its transcriptional coactivator activity. EP300. along with its paralog CBP (CREB-binding protein), is a histone acetyltransferase (HAT) that modulates chromatin structure and gene expression by acetylating histones and non-histone proteins. It plays essential roles in cell proliferation, differentiation, and apoptosis by interacting with transcription factors, including p53. NF-κB, and STATs. Phosphorylation at specific residues, such as S89. fine-tunes EP300's activity, stability, or interactions with partner proteins. The S89 phosphorylation site is implicated in cellular responses to DNA damage, growth signals, or stress, though its exact functional consequences remain under investigation. This antibody is widely used in research to study EP300 regulation in pathways like Wnt/β-catenin signaling, tumor suppression, and epigenetic reprogramming. Dysregulation of EP300 phosphorylation is linked to cancers, neurological disorders, and developmental defects. Validating the antibody's specificity via knockout controls or phosphatase treatment is recommended to ensure accurate detection in applications such as Western blotting, immunofluorescence, or ChIP assays.

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