WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/100-1/500 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Histone acetyltransferase p300, p300 HAT, E1A-associated protein p300, EP300, P300 |
Entrez GeneID | 2033 |
WB Predicted band size | 264.2kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This EP300 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S89 of human EP300. |
Formulation | Purified antibody in TBS with 0.05% sodium azide. |
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以下是关于Phospho-EP300(S89)抗体的假设性参考文献示例(仅供参考,具体文献需根据实际研究查询):
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1. **文献名称**:*Phosphorylation of EP300 at Serine 89 Mediates DNA Damage Response*
**作者**:Smith J, et al.
**摘要**:本研究揭示了EP300在DNA损伤后被ATM激酶磷酸化(S89位点),促进其与BRCA1复合物的结合,从而增强同源重组修复能力,维持基因组稳定性。
2. **文献名称**:*Oncogenic Role of p-EP300(S89) in Hepatocellular Carcinoma*
**作者**:Zhang L, et al.
**摘要**:通过免疫组化分析发现,Phospho-EP300(S89)在肝癌组织中高表达,其磷酸化水平与患者预后不良相关,可能通过激活Wnt/β-catenin通路促进肿瘤进展。
3. **文献名称**:*AMPK-Dependent Phosphorylation of EP300 at S89 Regulates Metabolic Adaptation*
**作者**:Lee H, et al.
**摘要**:研究发现AMPK在能量应激下磷酸化EP300(S89),抑制其组蛋白乙酰转移酶活性,从而调控糖异生相关基因表达,影响细胞代谢重编程。
4. **文献名称**:*Dynamic Phosphorylation of EP300 at Ser89 Modulates Cell Cycle Progression*
**作者**:Johnson R, et al.
**摘要**:S89位点的磷酸化在G1/S期转换中显著上调,通过调控p21和Cyclin D1的转录影响细胞周期进程,提示其在癌症治疗中的潜在靶点价值。
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**注意**:以上为基于EP300功能研究的假设性文献,实际研究中需通过数据库(如PubMed)以关键词“Phospho-EP300 Ser89”或“EP300 S89 phosphorylation”查询具体文献。
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