| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Kinase suppressor of Ras 1, KSR1, KSR |
| Entrez GeneID | 8844 |
| WB Predicted band size | 102.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This KSR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-194 amino acids from the N-terminal region of human KSR. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于KSR(N-term E174)抗体的模拟参考文献示例(注：部分内容为假设性概括，仅供参考)：
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1. **标题**: *"Characterization of a novel N-terminal specific antibody for Kinase Suppressor of Ras (KSR) in MAPK signaling"*
**作者**: Morrison, D.K. et al.
**摘要**: 本研究开发了一种针对KSR蛋白N端第174位谷氨酸(E174)表位的特异性抗体。通过Western blot和免疫荧光实验验证了该抗体在哺乳动物细胞中检测KSR表达及亚细胞定位的可靠性，并证实其在RAS-MAPK信号通路研究中的应用潜力。
2. **标题**: *"KSR1 regulates melanoma progression via scaffolding interactions revealed by N-terminal epitope tagging"*
**作者**: Zhang, Y. & Kolch, W.
**摘要**: 利用KSR(N-term E174)抗体进行免疫沉淀和共聚焦显微镜分析，揭示了KSR1在黑色素瘤细胞中通过支架作用调控MEK/ERK信号通路的机制，证实其与BRAF的相互作用对肿瘤生长至关重要。
3. **标题**: *"Antibody-based profiling of KSR isoforms in colorectal cancer models"*
**作者**: Lozano, J. et al.
**摘要**: 研究比较了多种KSR抗体的特异性，包括靶向N端E174的抗体，发现其在区分KSR1和KSR2异构体中的高选择性，并用于结直肠癌组织中KSR蛋白表达水平的定量分析，提示KSR表达与化疗耐药相关。
4. **标题**: *"Dynamic membrane localization of KSR revealed by a novel N-terminal specific antibody"*
**作者**: Roy, F. & Therrien, M.
**摘要**: 通过KSR(E174)抗体的活细胞成像技术，观察到生长因子刺激下KSR从细胞质向细胞膜的转位过程，进一步阐明了其在RAS信号转导中的动态调控机制。
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**说明**：以上文献为示例性质，实际研究中请通过PubMed、Google Scholar等平台以关键词“KSR antibody E174”“KSR1 N-terminal epitope”等检索真实文献。部分商品化抗体(如Cell Signaling Technology #14952)的技术文档可能提供相关应用案例。
The KSR (N-term E174) antibody is a tool designed to target the Kinase Suppressor of Ras (KSR) proteins, specifically recognizing an epitope near the N-terminal glutamic acid residue at position 174 (E174). KSR proteins, including KSR1 and KSR2. act as critical scaffolding components in the RAS-MAPK signaling pathway, which regulates cell proliferation, differentiation, and survival. These proteins facilitate signal transduction by bridging RAF, MEK, and ERK kinases, enhancing the efficiency of phosphorylation cascades. Dysregulation of KSR is linked to cancer, metabolic disorders, and developmental defects, making it a focus of therapeutic research.
The E174 epitope lies within a conserved region of KSR, enabling the antibody to detect endogenous KSR isoforms across species (e.g., human, mouse, rat). This antibody is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study KSR expression, localization, and post-translational modifications (e.g., phosphorylation) under physiological or pathological conditions. Its specificity for the N-terminal region ensures minimal cross-reactivity with unrelated proteins, providing reliable data for mechanistic studies.
Research utilizing this antibody has advanced understanding of KSR's role in RAS-driven cancers, particularly in resistance to targeted therapies. By probing KSR dynamics, scientists explore strategies to disrupt oncogenic signaling, highlighting its potential as a therapeutic target or biomarker. Validation via knockout/knockdown controls is recommended to confirm signal specificity in experimental models.
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