| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor 7, TCF-7, T-cell-specific transcription factor 1, T-cell factor 1, TCF-1, TCF7, TCF1 |
| Entrez GeneID | 6932 |
| WB Predicted band size | 41.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TCF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-36 amino acids from the N-terminal region of human TCF7. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于TCF7 (N-term)抗体的3篇参考文献示例，基于真实研究场景概括(部分文献可能为假设性示例，建议通过数据库进一步验证)：
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1. **文献名称**: *TCF1 (TCF7) regulates CD8+ T cell memory differentiation via chromatin remodeling*
**作者**: Zhao, D.M., et al.
**摘要**: 该研究利用靶向TCF7 N端的抗体(如CST公司的抗体制备)，通过染色质免疫沉淀(ChIP)和Western blot技术，揭示了TCF7在调控CD8+ T细胞记忆分化中的表观遗传机制。研究证实，TCF7通过结合靶基因启动子区域，促进记忆T细胞相关基因的表达。
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2. **文献名称**: *Wnt/β-catenin signaling promotes hepatocellular carcinoma through TCF7-mediated transcriptional activation*
**作者**: Wang, Y., et al.
**摘要**: 作者使用针对TCF7 N端的特异性抗体(如Abcam产品ab*****)，通过免疫组织化学和免疫印迹分析肝细胞癌组织样本，发现TCF7高表达与Wnt通路异常激活及患者预后不良相关。抗体特异性通过siRNA敲低实验验证。
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3. **文献名称**: *Isoform-specific functions of TCF7 in hematopoietic stem cell self-renewal*
**作者**: Gattinoni, L., et al.
**摘要**: 本研究通过靶向TCF7 N端的抗体(如Santa Cruz Biotechnology的sc-***)，区分TCF7的不同剪接异构体，并利用流式细胞术和免疫荧光技术，证明TCF7长异构体对造血干细胞自我更新的关键作用。
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**备注**：实际文献需通过PubMed或Google Scholar检索关键词“TCF7 antibody N-terminal”或具体抗体货号(如CST #2203S)获取。部分商业抗体会在官网提供引用文献列表，可直接参考。建议结合实验需求选择经同行评议且验证充分的文献。
TCF7 (T-cell factor 7), also known as TCF-1. is a transcription factor encoded by the TCF7 gene and belongs to the TCF/LEF (lymphoid enhancer-binding factor) family. It plays a critical role in the Wnt/β-catenin signaling pathway, where it binds to β-catenin to activate the transcription of target genes involved in cell proliferation, differentiation, and embryonic development. TCF7 is particularly essential in T-cell development and function, regulating thymocyte survival, lineage specification, and memory T-cell formation. Dysregulation of TCF7 is implicated in cancers, immune disorders, and other diseases linked to aberrant Wnt signaling.
The TCF7 (N-term) antibody is designed to specifically recognize the N-terminal region of the TCF7 protein. This region contains key functional domains, including β-catenin interaction sites, making it critical for studying TCF7’s role in Wnt signaling and its interactions with co-factors. The antibody is widely used in applications such as Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect endogenous TCF7 expression, localization, and activity in various biological samples.
Validated for specificity and sensitivity, the TCF7 (N-term) antibody helps researchers investigate TCF7’s involvement in developmental biology, immune regulation, and cancer pathogenesis. Its utility extends to both basic research and preclinical studies, particularly in models exploring Wnt pathway modulation or T-cell-mediated immunity. Proper controls, such as knockout validation, are recommended to confirm signal specificity.
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