| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 60S ribosomal export protein NMD3, hNMD3, NMD3 |
| Entrez GeneID | 51068 |
| WB Predicted band size | 57.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NMD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-440 amino acids from the C-terminal region of human NMD3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3-4条关于NMD3抗体的虚构参考文献(仅供示例，非真实文献)：
1. **标题**：*NMD3 Antibody Characterization in Ribosomal Subunit Export Studies*
**作者**：Smith, J. et al.
**摘要**：研究验证了一种高特异性NMD3抗体的应用，通过免疫印迹和免疫荧光证实其在检测真核细胞中NMD3蛋白定位及与核糖体大亚基转运相关的功能。
2. **标题**：*Role of NMD3 in Cancer: Insights from Antibody-Based Inhibition*
**作者**：Chen, L. & Wang, H.
**摘要**：利用NMD3特异性抗体阻断其功能，发现NMD3缺失导致癌细胞核糖体合成受阻，提示其作为潜在肿瘤治疗靶点。
3. **标题**：*Development of a Monoclonal NMD3 Antibody for Diagnostic Applications*
**作者**：Johnson, R. et al.
**摘要**：报道了一种新型单克隆抗体的开发，该抗体可特异性识别NMD3蛋白C端结构域，适用于组织样本中NMD3表达水平的定量分析。
4. **标题**：*NMD3-Mediated Ribosomal Export in Neurodegeneration: Antibody-Based Analysis*
**作者**：Garcia, M. et al.
**摘要**：通过NMD3抗体研究其在神经细胞中的表达异常，发现NMD3功能失调与阿尔茨海默病模型中核糖体组装缺陷相关。
(注：以上文献为模拟内容，实际引用时请查询真实数据库如PubMed、Google Scholar。)
The NMD3 antibody is a research tool used to study the NMD3 protein, a conserved factor critical in ribosome biogenesis and nuclear export of the 60S ribosomal subunit. In eukaryotes, NMD3 acts as a chaperone that facilitates the maturation and quality control of the 60S subunit within the nucleus before its transport to the cytoplasm. It binds to nascent 60S particles, recruits the export receptor Crm1/Xpo1 via a leucine-rich nuclear export signal (NES), and ensures proper assembly of ribosomal proteins and RNA. Dysregulation of NMD3 is linked to ribosomopathies and cancers, as altered ribosome biogenesis can disrupt cellular proliferation.
The NMD3 antibody is widely employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect NMD3 expression, localization, and interactions. Researchers use it to investigate ribosome assembly defects, nucleocytoplasmic transport mechanisms, and diseases associated with ribosomal dysfunction. Studies in yeast, mammalian cells, and model organisms highlight its conserved role in ribosome export. Commercial NMD3 antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions, and validation includes testing in knockdown models or functional assays to confirm specificity. This antibody remains pivotal in exploring ribosome biology and related pathologies.
×
