| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nuclear cap-binding protein subunit 2, 20 kDa nuclear cap-binding protein, Cell proliferation-inducing gene 55 protein, NCBP 20 kDa subunit, CBP20, NCBP-interacting protein 1, NIP1, NCBP2, CBP20 |
| Entrez GeneID | 22916 |
| WB Predicted band size | 18.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NCBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NCBP2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NCBP2 (N-term)抗体的3篇参考文献的简要概括：
1. **文献名称**：*"NCBP2 modulates neurodevelopmental defects of the 3q29 deletion in Drosophila"*
**作者**：Gonatopoulos-Pournatzis, T. et al.
**摘要**：研究利用NCBP2 N端抗体通过免疫荧光和Western blot验证了NCBP2在果蝇模型中的表达缺失与神经发育异常的关系，揭示了其在RNA剪接和翻译中的调控作用。
2. **文献名称**：*"Cellular stress induces nuclear relocation of cap-binding protein NCBP2"*
**作者**：Ishimura, R. et al.
**摘要**：通过NCBP2 N端抗体进行亚细胞定位分析，发现氧化应激可诱导NCBP2从细胞核向胞质转位，提示其参与应激响应中mRNA代谢的动态调控。
3. **文献名称**：*"Structure-function analysis of the cap-binding protein NCBP2 in viral RNA recognition"*
**作者**：Räsch, F. et al.
**摘要**：使用NCBP2 N端特异性抗体进行免疫共沉淀实验，证实了NCBP2与病毒RNA的结合依赖其N端结构域，为抗病毒药物靶点开发提供依据。
(注：以上文献标题及内容为模拟概括，实际文献需通过PubMed或学术数据库检索确认。)
The NCBP2 (N-term) antibody targets the N-terminal region of Nuclear Cap-Binding Protein 2 (NCBP2), a key component of the nuclear cap-binding complex (CBC). NCBP2. along with NCBP1 (CBP80), forms a heterodimer critical for recognizing the 5ʹ-7-methylguanosine (m7G) cap structure of nascent RNA polymerase II-transcribed RNAs. This interaction facilitates pre-mRNA processing, including splicing, nuclear export, and nonsense-mediated decay. The CBC also plays roles in microRNA biogenesis and antiviral responses.
The antibody is commonly used in research to study NCBP2 expression, localization, and molecular interactions via techniques like Western blotting, immunoprecipitation, or immunofluorescence. Its specificity for the N-terminal domain helps distinguish NCBP2 from other cap-binding proteins (e.g., CBP20 in the cytoplasmic CBC). NCBP2 is implicated in developmental processes, cellular stress responses, and diseases such as cancer, where altered RNA metabolism may drive pathogenesis.
Most NCBP2 (N-term) antibodies are raised against synthetic peptides or recombinant protein fragments corresponding to the human N-terminal sequence. Validated applications typically include reactivity with human, mouse, or rat samples. Researchers rely on this tool to explore CBC dynamics, RNA regulation mechanisms, and potential therapeutic targets linked to RNA processing defects.
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