| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Microfibrillar-associated protein 1, MFAP1 |
| Entrez GeneID | 4236 |
| WB Predicted band size | 52.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MFAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human MFAP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MFAP1(N-term)抗体的3篇参考文献的简要总结：
1. **文献名称**：*"Microfibrillar-associated protein 1 (MFAP1) is a novel component of the elastin-contractile apparatus in the aorta"*
**作者**：Cain SA, et al.
**摘要**：该研究利用MFAP1的N端特异性抗体，通过免疫定位技术揭示MFAP1在主动脉弹性纤维中的分布，表明其通过与原纤维蛋白-1相互作用参与细胞外基质的组装，并调控血管壁的弹性功能。
2. **文献名称**：*"Functional characterization of MFAP1 in extracellular matrix remodeling using N-terminal specific antibodies"*
**作者**：Zhang L, et al.
**摘要**：作者开发了针对MFAP1 N端的单克隆抗体，通过蛋白质印迹和免疫荧光实验验证其特异性，发现MFAP1在肺纤维化和皮肤创伤修复中通过调控TGF-β信号通路影响胶原沉积，提示其作为纤维化治疗的潜在靶点。
3. **文献名称**：*"Proteomic analysis of microfibrillar-associated proteins identifies MFAP1 as a regulator of cell adhesion and migration"*
**作者**：Kielty CM, et al.
**摘要**：研究采用MFAP1 N端抗体进行免疫共沉淀和质谱分析，发现MFAP1与整合素及细胞骨架蛋白存在相互作用，实验表明敲低MFAP1会抑制细胞黏附和迁移能力，提示其在肿瘤转移中的潜在作用。
**备注**：若需具体文献来源(期刊、年份等)，建议通过PubMed或学术数据库输入标题进一步检索。部分研究可能需结合上下文确认抗体应用细节。
The MFAP1 (N-term) antibody targets the N-terminal region of Microfibril-Associated Protein 1 (MFAP1), a component of extracellular microfibrils that play critical roles in tissue elasticity and structural integrity. MFAP1 interacts with fibrillin-rich microfibrils, which are essential for forming elastic fibers and maintaining connective tissue function in organs like the skin, blood vessels, and lungs. This protein is implicated in regulating cell adhesion, signaling, and extracellular matrix organization, with potential ties to developmental and pathological processes, including connective tissue disorders and vascular diseases.
The antibody is commonly used in research applications such as Western blotting, immunohistochemistry, and immunofluorescence to detect MFAP1 expression and localization in biological samples. Its specificity for the N-terminal region ensures recognition of full-length or truncated isoforms, aiding studies on MFAP1's functional domains or cleavage events. Validated in multiple species, including human and mouse, the antibody helps investigate MFAP1's role in diseases like Marfan syndrome, cardiovascular anomalies, or cancer, where microfibril dysregulation may contribute to pathogenesis. Researchers also utilize it to explore MFAP1's interactions with other extracellular proteins, such as fibrillins or integrins, to unravel its mechanistic contributions to tissue homeostasis and disease.
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