| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PR65B; PP2A-Abeta |
| WB Predicted band size | 66 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PPP2R1B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于Phospho-Rb(S811)抗体的3篇参考文献示例(文献为虚构，仅作格式参考)：
1. **文献名称**: *Cyclin-Dependent Kinase Regulation of Rb Phosphorylation in Cell Cycle Progression*
**作者**: Smith J, et al.
**摘要**: 研究利用Phospho-Rb(S811)抗体揭示CDK4/6在G1期特异性磷酸化Rb蛋白S811位点，促进细胞周期进入S期的分子机制。
2. **文献名称**: *DNA Damage-Induced Rb Inactivation via ATM/ATR Signaling*
**作者**: Lee H, et al.
**摘要**: 通过Phospho-Rb(S811)抗体检测，发现DNA损伤后ATM/ATR激酶激活导致Rb S811位点磷酸化，阻断其与E2F结合，触发细胞周期停滞。
3. **文献名称**: *Phospho-Rb(S811) as a Biomarker in Breast Cancer Prognosis*
**作者**: Garcia R, et al.
**摘要**: 该研究使用Phospho-Rb(S811)抗体分析乳腺癌组织样本，发现S811磷酸化水平与肿瘤侵袭性及患者预后不良显著相关。
(注：以上文献为模拟生成，实际引用需通过PubMed/Google Scholar检索关键词“Phospho-Rb S811 antibody”或相关研究领域筛选。)
The Phospho-Rb(S811) antibody detects the retinoblastoma protein (Rb) when phosphorylated at serine 811. a key regulatory site in cell cycle control. Rb, a tumor suppressor, regulates the G1-S phase transition by binding and inhibiting E2F transcription factors. Phosphorylation of Rb, particularly at S811 (mediated by cyclin-dependent kinases, CDKs), disrupts this interaction, releasing E2F to activate genes essential for DNA replication and cell cycle progression. This phosphorylation event is a hallmark of cell cycle commitment and is tightly regulated in normal cells. Dysregulation of Rb phosphorylation is linked to uncontrolled proliferation in cancers.
The Phospho-Rb(S811) antibody is widely used in research to study cell cycle dynamics, checkpoint mechanisms, and oncogenic signaling. It helps assess Rb inactivation status in cancer models, drug screening (e.g., CDK inhibitors), and studies of cellular senescence or differentiation. Detection methods include Western blotting, immunofluorescence, and immunohistochemistry using cell lysates or tissue samples. Specificity for the phosphorylated epitope ensures accurate monitoring of Rb activity, making it a critical tool for understanding Rb-dependent pathways in health and disease. Validation often involves phosphorylation-specific controls or CDK inhibitor-treated samples to confirm signal modulation.
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