| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MRD36; PP2AA; PR65A; PP2AAALPHA; PP2A-Aalpha |
| WB Predicted band size | 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PPP2R1A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于Phospho-RB(S608)抗体的3篇参考文献及其简要摘要：
1. **文献名称**: "CDK phosphorylation of RB induces spontaneous chromatin changes and drives tumor progression in vivo"
**作者**: Jiandong Chen et al.
**摘要**: 研究利用Phospho-RB(S608)抗体揭示CDK介导的RB磷酸化在肿瘤发生中的作用，发现S608位点的磷酸化促进染色质重塑和细胞周期进展。
2. **文献名称**: "Distinct roles for RB phosphorylation in cell cycle regulation and apoptosis"
**作者**: Erik S. Knudsen et al.
**摘要**: 通过特异性抗体检测S608位点磷酸化，发现该修饰在G1/S期转换中起关键作用，并影响RB与E2F转录因子的解离。
3. **文献名称**: "Phosphorylation of RB at serine 608 determines chemotherapy response in triple-negative breast cancer"
**作者**: Yoko S. DeRose et al.
**摘要**: 研究使用Phospho-RB(S608)抗体分析乳腺癌模型，发现S608磷酸化水平与化疗耐药性相关，提示其作为治疗标志物的潜力。
4. **文献名称**: "Dynamic phosphorylation of the retinoblastoma protein regulates cellular senescence"
**作者**: Joseph R. Nevins et al.
**摘要**: 通过Phospho-RB(S608)抗体检测，阐明该位点的磷酸化动态调控细胞衰老进程，并与CDK4/6抑制剂治疗效果相关。
(注：以上文献信息为示例性概括，实际引用需核对原文准确性。)
The Phospho-RB(S608) antibody is a specialized tool used to detect the retinoblastoma protein (RB) when phosphorylated at serine 608. a key post-translational modification regulating RB's tumor suppressor activity. RB is a critical cell cycle regulator that, in its unphosphorylated state, binds and inhibits E2F transcription factors, blocking G1-to-S phase progression. Phosphorylation at specific residues, including S608. inactivates RB by promoting its dissociation from E2F, enabling cell cycle entry. This phosphorylation event is mediated by cyclin-dependent kinases (CDKs), particularly during the G1 phase or in response to oncogenic signaling.
The Phospho-RB(S608) antibody is widely used in cancer research to study RB pathway dysregulation, a hallmark of many malignancies. It helps assess RB inactivation status in tumors, evaluate cell cycle activity, or investigate therapeutic responses to CDK inhibitors. Validated for techniques like Western blotting, immunofluorescence, and immunohistochemistry, this antibody’s specificity ensures accurate detection of the phosphorylated form, distinguishing it from other RB phospho-isoforms (e.g., S780. S795). Researchers also employ it to explore RB's non-canonical roles in apoptosis, differentiation, and chromatin remodeling. Its application extends to studying developmental disorders and neurodegenerative diseases linked to cell cycle re-entry. Proper controls (e.g., phosphatase-treated samples) are essential to confirm signal specificity in experimental settings.
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