| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAP-K5, MAPKAPK-5, MK-5, MK5, p38-regulated/activated protein kinase, PRAK, MAPKAPK5, PRAK |
| Entrez GeneID | 8550 |
| WB Predicted band size | 54.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This MAPKAPK5 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S93 of human MAPKAPK5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-MAPKAPK5(S93)抗体的模拟参考文献示例(仅供格式参考，具体内容需查阅真实文献)：
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1. **文献名称**：*Phosphorylation of MAPKAPK5 at Ser93 regulates inflammatory cytokine production in macrophages*
**作者**：J. Han et al.
**摘要**：研究揭示了MAPKAPK5在p38 MAPK通路中的磷酸化位点S93对巨噬细胞炎症因子(如TNF-α、IL-6)分泌的调控作用，并通过该抗体验证了LPS刺激下的磷酸化动态变化。
2. **文献名称**：*MAPKAPK5 activation via S93 phosphorylation promotes cancer cell migration and invasion*
**作者**：C. D. Müller et al.
**摘要**：本文利用Phospho-MAPKAPK5(S93)抗体证实，在乳腺癌细胞中，S93位点的磷酸化通过增强激酶活性促进细胞迁移和侵袭，并与患者预后不良相关。
3. **文献名称**：*Structural basis of MAPKAPK5 activation by phosphorylation at Ser93*
**作者**：K. Yamaguchi et al.
**摘要**：通过晶体结构分析和抗体检测，揭示了S93磷酸化诱导MAPKAPK5构象变化，从而促进其与下游底物(如HSP27)结合的分子机制。
4. **文献名称**：*Oxidative stress triggers MAPKAPK5 phosphorylation at Ser93 to regulate autophagy*
**作者**：A. R. Bennett et al.
**摘要**：研究发现氧化应激通过激活p38 MAPK通路诱导MAPKAPK5(S93)磷酸化，进而调控自噬相关蛋白的活性，该抗体被用于体内外模型的磷酸化水平检测。
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**注**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“MAPKAPK5 S93 phosphorylation”或抗体货号)获取。建议结合具体研究场景选择近年高引论文。
**Background of Phospho-MAPKAPK5 (S93) Antibody**
Phospho-MAPKAPK5 (S93) antibody is a specialized tool used to detect the phosphorylated form of MAPKAPK5 (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5) at serine residue 93. MAPKAPK5. also known as PRAK, is a serine/threonine kinase downstream of the p38 MAPK signaling pathway. Its activation occurs via phosphorylation by p38 MAPK in response to cellular stressors, cytokines, or growth factors, enabling its role in regulating diverse processes like inflammation, cell proliferation, and apoptosis.
The S93 phosphorylation site is critical for MAPKAPK5 activation, marking its transition to an enzymatically active state. This antibody specifically recognizes the phosphorylated S93 epitope, allowing researchers to study the kinase's activation status under experimental conditions such as oxidative stress, heat shock, or inflammatory stimuli.
Applications of this antibody include Western blotting, immunofluorescence, and immunohistochemistry, aiding in the investigation of MAPKAPK5's involvement in diseases like cancer, neurodegenerative disorders, and cardiovascular pathologies. Its specificity helps dissect signaling crosstalk in pathways linked to p38 MAPK, ERK, or PI3K/AKT, offering insights into therapeutic targeting. Validation often involves knockout controls or phosphatase treatment to confirm phosphorylation-dependent detection. Overall, this antibody serves as a key reagent for exploring MAPKAPK5's functional dynamics in health and disease.
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