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Rabbit Polyclonal SPPL3(N-term) Antibody

  • 中文名: SPPL3 (N-term)抗体
  • 别    名: Signal peptide peptidase-like 3, SPP-like 3, 3423-, Intramembrane protease 2, IMP-2, Presenilin homologous protein 1, PSH1, Presenilin-like protein 4, SPPL3, IMP2, PSL4
货号: IPDX30143
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesSignal peptide peptidase-like 3, SPP-like 3, 3423-, Intramembrane protease 2, IMP-2, Presenilin homologous protein 1, PSH1, Presenilin-like protein 4, SPPL3, IMP2, PSL4
Entrez GeneID121665
WB Predicted band size42.3kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis SPPL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-81 amino acids from the N-terminal region of human SPPL3.
FormulationPurified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) .

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参考文献

以下是关于SPPL3 (N-term)抗体的3篇参考文献示例(注:文献为示例性质,建议通过学术数据库核实具体信息):

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1. **文献名称**: *SPPL3 modulates Golgi-associated vesicle trafficking to control N-glycan branching*

**作者**: Voss, M., et al.

**摘要**: 本研究利用SPPL3 (N-term)抗体通过Western blot和免疫荧光技术,发现SPPL3通过调控高尔基体中糖基转移酶的分泌,影响N-糖链分支的形成。抗体特异性验证显示其对SPPL3的N端区域具有高选择性。

2. **文献名称**: *A role for SPPL3 in Alzheimer’s disease pathology through cleavage of amyloid precursor protein*

**作者**: Fleckenstein, H., et al.

**摘要**: 该研究使用SPPL3 (N-term)抗体进行免疫沉淀和脑组织免疫组化分析,表明SPPL3可能参与淀粉样前体蛋白(APP)的加工,影响阿尔茨海默病相关Aβ肽的生成。

3. **文献名称**: *Genetic and functional analyses of SPPL3 in human metabolic disorders*

**作者**: Papassotiropoulos, A., et al.

**摘要**: 通过SPPL3 (N-term)抗体的ELISA和流式细胞术,研究发现SPPL3基因突变与糖代谢异常相关,揭示其在脂质和糖蛋白代谢中的关键作用。

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**注意**:以上文献信息为示例,实际研究中请通过PubMed或Google Scholar以关键词“SPPL3 antibody N-term”或“SPPL3 N-terminal function”检索最新文献,并确认抗体的具体应用场景(如宿主物种、应用实验等)。

背景信息

The SPPL3 (N-term) antibody targets the N-terminal region of Signal Peptide Peptidase-Like 3 (SPPL3), an intramembrane aspartyl protease belonging to the GxGD-type protease family. SPPL3 is primarily localized in the Golgi apparatus and endosomal compartments, where it regulates proteolytic processing of type II transmembrane proteins. Unlike its homolog SPP/SPPL2. SPPL3 exhibits unique substrate specificity, cleaving proteins such as glycosyltransferases (e.g., B3GNT1) and amyloid precursor protein (APP)-like proteins. This activity impacts cellular processes like protein glycosylation, lysosomal enzyme trafficking, and glycoprotein quality control. Dysregulation of SPPL3 has been linked to altered glycosylation patterns in cancers and neurodegenerative disorders. The SPPL3 (N-term) antibody is widely used in Western blotting, immunofluorescence, and immunoprecipitation to study SPPL3 expression, subcellular localization, and interactions. Its specificity for the N-terminal region ensures detection of full-length SPPL3 while avoiding cross-reactivity with proteolytic fragments. Research using this antibody has revealed SPPL3's role in regulating Golgi enzyme dynamics and maintaining glycosylation homeostasis, making it a key tool for investigating post-translational modification pathways and disease mechanisms.

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