| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FAS-associated death domain protein, FAS-associating death domain-containing protein, Mediator of receptor induced toxicity, Protein FADD, Fadd, Mort1 |
| Entrez GeneID | 14082 |
| WB Predicted band size | 23.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This mouse FADD Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S191 of mouse FADD. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
+ +
以下是关于Phospho-mouse FADD(S191)抗体的假设参考文献示例，格式为文献名称、作者及摘要概括：
---
1. **文献名称**: *FADD phosphorylation at serine 191 regulates T-cell receptor-mediated apoptosis*
**作者**: Zhang, J., et al.
**摘要**: 该研究利用Phospho-mouse FADD(S191)抗体，发现T细胞激活过程中S191位点的磷酸化通过抑制FADD与caspase-8的结合，负调控死亡受体介导的凋亡信号通路。
2. **文献名称**: *Phosphorylation-dependent regulation of necroptosis by FADD in murine models*
**作者**: Lin, Y., et al.
**摘要**: 通过特异性抗体检测小鼠FADD(S191)磷酸化，揭示该修饰在坏死性凋亡中的关键作用，表明S191磷酸化促进RIPK1-RIPK3复合体形成，驱动炎症性细胞死亡。
3. **文献名称**: *FADD S191 phosphorylation confers resistance to apoptosis in colorectal cancer*
**作者**: Wang, H., et al.
**摘要**: 使用Phospho-FADD(S191)抗体分析临床样本，发现结直肠癌细胞中S191的高磷酸化水平与化疗耐药性相关，机制涉及凋亡信号通路的异常抑制。
4. **文献名称**: *Characterization of a phospho-specific antibody for mouse FADD Ser191 and its application in immunofluorescence*
**作者**: Smith, L.M., et al.
**摘要**: 本文验证了Phospho-mouse FADD(S191)抗体的特异性，展示其在免疫荧光和免疫印迹中的应用，并发现该位点磷酸化在细胞周期调控中的潜在作用。
---
**备注**：上述文献为示例性质，实际引用需通过PubMed、Google Scholar等平台以关键词“Phospho-FADD S191 mouse”“FADD phosphorylation Ser191”检索真实研究。部分商业抗体厂商(如Cell Signaling Technology)的产品说明页也可能提供相关参考文献。
The Phospho-mouse FADD (Serine 191) antibody is specifically designed to detect FADD (Fas-associated protein with death domain) when phosphorylated at serine residue 191 in mouse models. FADD is a critical adaptor protein involved in death receptor-mediated apoptotic signaling, linking activated death receptors like Fas to initiator caspases (e.g., caspase-8/10) through its death effector domain (DED). While FADD is traditionally associated with apoptosis, phosphorylation at specific residues, including S191. has been implicated in non-apoptotic functions, such as regulating cell cycle progression, proliferation, and immune responses.
In T-cells, phosphorylation of FADD at S191 occurs during T-cell receptor (TCR) activation and is mediated by kinases like casein kinase 1α (CK1α). This post-translational modification alters FADD’s conformation, potentially shifting its role from pro-apoptotic to pro-survival signaling by modulating interactions with other DED-containing proteins or disrupting caspase recruitment. Studies using this antibody have helped elucidate FADD’s dual roles in apoptosis and immune regulation, particularly in mouse models of inflammation, autoimmunity, and cancer.
The antibody is widely used in Western blotting, immunohistochemistry, and flow cytometry to study FADD phosphorylation dynamics in response to cellular stimuli or pathological conditions. Its specificity for the phosphorylated S191 epitope ensures accurate detection of this regulatory modification, aiding research into mechanisms underlying immune cell activation, tumor resistance, and therapeutic targeting of FADD-related pathways.
×
