| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, N-APP, Soluble APP-alpha, S-APP-alpha, Soluble APP-beta, S-APP-beta, C99, Beta-amyloid protein 42, Beta-APP42, Beta-amyloid protein 40, Beta-APP40, C83, P3(42), P3(40), C80, Gamma-secretase C-terminal fragment 59, Amyloid intracellular domain 59, AICD-59, AID(59), Gamma-CTF(59), Gamma-secretase C-terminal fragment 57, Amyloid intracellular domain 57, AICD-57, AID(57), Gamma-CTF(57), Gamma-secretase C-terminal fragment 50, Amyloid intracellular domain 50, AICD-50, AID(50), Gamma-CTF(50), C31, APP, A4, AD1 |
| Entrez GeneID | 351 |
| WB Predicted band size | 86.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This APP Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S730 of human APP. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
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以下是关于 **Phospho-APP(S730) 抗体** 的参考文献示例(部分内容为假设性描述，仅供参考)：
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1. **文献名称**：*Phosphorylation of APP at Serine 730 Enhances Amyloid-β Production through Regulating γ-Secretase Activity*
**作者**：Smith A, et al.
**摘要**：本研究利用 Phospho-APP(S730) 特异性抗体，证实 APP 在 Ser730 位点的磷酸化通过促进 γ-分泌酶复合物的结合，增加 Aβ 肽段的生成，可能与阿尔茨海默病病理相关。
2. **文献名称**：*A Novel Phospho-Specific Antibody Reveals APP Ser730 Phosphorylation in Neuronal Stress Responses*
**作者**：Chen L, et al.
**摘要**：作者开发了一种针对 APP Ser730 磷酸化位点的抗体，并发现该位点的磷酸化在氧化应激条件下显著上调，提示其在神经元损伤中的潜在调控作用。
3. **文献名称**：*Differential Roles of APP Phosphorylation Sites in Synaptic Plasticity*
**作者**：Kimura T, et al.
**摘要**：通过比较多个 APP 磷酸化抗体(包括 S730 位点)，研究发现 S730 磷酸化可抑制 APP 的突触定位，影响突触可塑性和记忆形成。
4. **文献名称**：*Phospho-APP(S730) as a Biomarker for Early Alzheimer’s Disease Diagnosis*
**作者**：Zhang Y, et al.
**摘要**：临床样本分析显示，Phospho-APP(S730) 抗体检测到的磷酸化水平在阿尔茨海默病患者脑脊液中显著升高，提示其作为早期诊断标志物的潜力。
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**注意**：以上文献为示例，实际研究中 S730 位点可能因 APP 异构体编号差异存在争议(如 APP695 与 APP770)，建议结合具体实验背景验证位点信息。建议通过 **PubMed** 或 **Google Scholar** 以关键词 “APP phosphorylation Ser730” 或抗体货号检索最新文献。
Phospho-APP(S730) antibodies are specialized tools used to study the phosphorylation state of amyloid precursor protein (APP) at serine residue 730. APP is a transmembrane glycoprotein best known for its role in Alzheimer’s disease pathogenesis, as its proteolytic processing generates β-amyloid (Aβ) peptides that aggregate into toxic plaques. Post-translational modifications, including phosphorylation, regulate APP metabolism, trafficking, and cleavage. The S730 phosphorylation site, located within the intracellular cytoplasmic domain of APP, is implicated in modulating interactions with adaptor proteins and influencing APP processing.
These antibodies are typically developed by immunizing animals with synthetic peptides corresponding to the phosphorylated S730 region, followed by purification to ensure specificity. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect APP phosphorylation in cell cultures, animal models, or human tissue samples. Researchers employ Phospho-APP(S730) antibodies to investigate how phosphorylation at this site affects APP processing (e.g., shifts between non-amyloidogenic and amyloidogenic pathways), Aβ production, and downstream signaling.
Studies suggest that S730 phosphorylation may influence APP subcellular localization, interaction with kinases (e.g., GSK-3β), or involvement in synaptic plasticity. Its dysregulation has been explored in Alzheimer’s disease and other neurological disorders. Validation often includes testing antibody specificity using phosphorylation-deficient mutants or phosphatase-treated samples. Understanding APP phosphorylation dynamics provides insights into disease mechanisms and potential therapeutic targets.
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