| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Glutathione S-transferase A3, GST class-alpha member 3, Glutathione S-transferase Ya3, Glutathione S-transferase Yc, Gsta3, Gstyc |
| Entrez GeneID | 14859 |
| WB Predicted band size | 25.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Gsta3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-211 amino acids from the C-terminal region of mouse Gsta3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Phospho-ABL1(Y134)抗体的模拟参考文献(注：部分文献信息为示例性构建，建议通过PubMed等数据库验证真实文献)：
1. **"ABL1 tyrosine 134 phosphorylation regulates kinase activation and substrate specificity"**
*作者：Smith J, et al. (2018)*
摘要：本研究通过质谱分析发现ABL1在Y134位点的磷酸化可增强其激酶活性，并开发了特异性Phospho-ABL1(Y134)抗体。该抗体在慢性髓性白血病(CML)细胞模型中验证了其检测能力，并揭示Y134磷酸化对BCR-ABL1信号通路的调控作用。
2. **"Development of a phosphospecific antibody for ABL1 Y134 and its application in solid tumors"**
*作者：Li X, et al. (2020)*
摘要：作者报道了一种高特异性Phospho-ABL1(Y134)多克隆抗体的制备方法，并通过免疫印迹和免疫组化验证其在结直肠癌组织中的表达。研究提示Y134磷酸化可能与ABL1的非经典致癌途径相关。
3. **"Y134 phosphorylation of ABL1 promotes its interaction with CrkL in cellular stress response"**
*作者：Garcia R, et al. (2021)*
摘要：利用Phospho-ABL1(Y134)抗体，本研究证明DNA损伤条件下Y134磷酸化介导ABL1与适配蛋白CrkL的结合，进而激活下游JNK信号通路，为ABL1在应激反应中的功能提供了新机制。
4. **"Comparative analysis of ABL1 phosphorylation sites using phospho-specific antibodies"**
*作者：Wang H, et al. (2019)*
摘要：系统比较了包括Y134在内的多个ABL1磷酸化位点抗体，确认Phospho-ABL1(Y134)抗体在区分不同激活状态下的特异性，并应用于伊马替尼耐药性CML患者的样本检测。
(注：以上文献为示例性内容，实际文献需通过学术数据库检索确认。)
The Phospho-ABL1(Y134) antibody is a specialized tool used to detect the phosphorylation status of tyrosine residue 134 (Y134) in the ABL1 (Abelson murine leukemia viral oncogene homolog 1) protein. ABL1 is a non-receptor tyrosine kinase involved in regulating cell proliferation, differentiation, and apoptosis. Its activity is tightly controlled by intramolecular interactions between its SH3. SH2. and kinase domains. Phosphorylation at Y134. located within the SH3 domain, disrupts these autoinhibitory interactions, leading to kinase activation. This modification can occur through autophosphorylation or via upstream kinases like SRC family kinases, enhancing ABL1's kinase activity and promoting interactions with downstream signaling partners.
Aberrant ABL1 activation, particularly through chromosomal translocations such as BCR-ABL1 in chronic myeloid leukemia (CML), drives uncontrolled cell growth. The Y134 phosphorylation site is critical for understanding ABL1's regulatory mechanisms and its oncogenic potential. The Phospho-ABL1(Y134) antibody is thus widely utilized in research to study ABL1 activation dynamics, signaling pathways, and responses to tyrosine kinase inhibitors (e.g., imatinib). It is validated for applications like Western blotting, immunoprecipitation, and immunohistochemistry, ensuring specificity for the phosphorylated epitope. This antibody serves as a key reagent in both basic research and translational studies, aiding in the exploration of ABL1-related malignancies and therapeutic resistance mechanisms.
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