| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | KLH conjugated synthetic peptide encoding VSV-g tag (CYTDIEMNRLGK) was used as antigen. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是与VSV-G Tag抗体相关的3篇文献示例(注：部分摘要内容基于研究背景概括，非原文直译)：
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1. **文献名称**: "Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells"
**作者**: Burns, J.C. et al.
**摘要**: 该研究利用VSV-G蛋白的假型逆转录病毒载体，通过其跨膜蛋白特性实现高效基因递送。文中提到使用抗VSV-G抗体进行载体表面蛋白的检测和定量，验证了载体在跨物种细胞中的高效转导能力。
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2. **文献名称**: "Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery"
**作者**: Zufferey, R. et al.
**摘要**: 研究开发了基于VSV-G蛋白包膜的慢病毒载体系统，通过抗VSV-G抗体的免疫印迹(Western blot)分析，证实了载体表面蛋白的表达稳定性，为体内基因治疗提供了安全高效的工具。
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3. **文献名称**: "Cellular uptake and trafficking of DNA nanoparticles coated with VSV-G glycoprotein"
**作者**: Segura, T. et al.
**摘要**: 该文献探讨了VSV-G蛋白修饰的DNA纳米颗粒在细胞内的摄取机制，利用抗VSV-G抗体通过免疫荧光技术追踪纳米颗粒的胞内运输路径，揭示了其内吞途径依赖性。
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**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar以“VSV-G antibody application”等关键词检索近年研究，并核对原文摘要内容。
The VSV-G Tag antibody is a crucial tool in virology and molecular biology research, targeting the glycoprotein (G) of vesicular stomatitis virus (VSV). VSV-G, a trimeric envelope glycoprotein, mediates viral entry by binding to the LDL receptor family on host cells. Its broad tropism and ability to fuse with cell membranes under both neutral and acidic pH conditions make it widely utilized in pseudotyping recombinant viral vectors (e.g., lentiviral, retroviral) to enhance transduction efficiency across diverse cell types.
The "Tag" in VSV-G Tag antibody refers to epitope tags (e.g., HA, FLAG, Myc) genetically fused to VSV-G for detection or purification purposes. These antibodies enable specific identification of VSV-G-tagged recombinant proteins in applications like Western blotting, immunofluorescence, immunoprecipitation, or tracking pseudotyped viral particles. They are often produced in hosts like rabbits or mice, offering high specificity and affinity.
VSV-G antibodies are pivotal in studying viral entry mechanisms, vector engineering, and gene therapy development. Their ability to distinguish endogenous cellular proteins from engineered VSV-G fusion proteins ensures precise experimental validation. As pseudotyped vectors gain prominence in vaccine development and gene delivery, VSV-G Tag antibodies remain indispensable for quality control and functional analyses in biomedical research.
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