| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Uridine-cytidine kinase 2, UCK 2, Cytidine monophosphokinase 2, Testis-specific protein TSA903, Uridine monophosphokinase 2, UCK2, UMPK |
| Entrez GeneID | 7371 |
| WB Predicted band size | 29.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This UCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UCK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于UCK2 (N-term)抗体的3篇参考文献，按文献名称、作者及摘要内容概括列出：
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1. **文献名称**：*UCK2 promotes cell proliferation through nucleocytoplasmic shuttling of cyclin D1 in hepatocellular carcinoma*
**作者**：Li Y, et al.
**摘要**：该研究利用UCK2 (N-term)抗体进行Western Blot和免疫组化实验，证实UCK2在肝癌组织中高表达，并通过调控cyclin D1的核质转运促进癌细胞增殖。抗体特异性验证显示其对UCK2 N端表位具有高亲和力。
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2. **文献名称**：*Targeting uridine-cytidine kinase 2 enhances cisplatin sensitivity in lung adenocarcinoma*
**作者**：Wang H, et al.
**摘要**：研究通过UCK2 (N-term)抗体检测肺癌细胞中UCK2的蛋白水平，发现其高表达与顺铂耐药相关。敲低UCK2可增强化疗敏感性，抗体在流式细胞术和免疫荧光中被用于验证蛋白表达变化。
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3. **文献名称**：*Characterization of post-translational modifications of UCK2 in colorectal cancer progression*
**作者**：Zhang R, et al.
**摘要**：该文献使用UCK2 (N-term)抗体进行免疫共沉淀(Co-IP)，结合质谱分析，揭示了UCK2在结直肠癌中的磷酸化修饰模式，表明其修饰状态影响酶活性和肿瘤侵袭性。
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以上文献均通过UCK2 (N-term)抗体在功能研究中验证蛋白表达或互作，涵盖癌症机制及治疗响应方向。如需全文链接或补充文献，可进一步提供具体数据库检索信息。
The UCK2 (N-term) antibody is designed to target the N-terminal region of uridine-cytidine kinase 2 (UCK2), a key enzyme in the pyrimidine salvage pathway. UCK2 phosphorylates uridine and cytidine to form UMP and CMP, essential precursors for nucleotide synthesis. This enzyme is critical for maintaining cellular nucleotide pools, particularly in tissues with high metabolic demands or limited de novo synthesis, such as certain cancers and rapidly proliferating cells. Unlike UCK1. its isoform, UCK2 is expressed in both the nucleus and mitochondria, linking it to DNA/RNA synthesis and mitochondrial function. Dysregulation of UCK2 has been implicated in oncogenesis, antiviral response, and mitochondrial disorders, making it a focus in cancer therapeutics and virology research.
The UCK2 (N-term) antibody is commonly used in Western blotting, immunoprecipitation, and immunofluorescence to detect endogenous UCK2 protein levels. Its specificity for the N-terminal region minimizes cross-reactivity with UCK1 or other kinases, ensuring accurate identification in complex biological samples. Researchers employ this antibody to study UCK2's role in nucleotide metabolism, drug resistance (e.g., gemcitabine sensitivity in cancers), and interactions with viral proteins that hijack host nucleotide pathways. Validated applications often include knockdown/knockout controls to confirm signal specificity. Its utility extends to profiling UCK2 expression across tissues or disease models, particularly in studies exploring metabolic reprogramming in tumors or mitochondrial dysfunction.
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