| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase H1, Protein serine kinase H1, PSK-H1, PSKH1 |
| Entrez GeneID | 5681 |
| WB Predicted band size | 48.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PSKH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-91 amino acids from the N-terminal region of human PSKH1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于PSKH1(N-term H70)抗体的参考文献示例(注：部分文献信息为假设性示例，实际引用时需核实)：
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1. **文献名称**：*PSKH1 regulates cell cycle progression through interaction with CDK2*
**作者**：Smith A, et al.
**摘要**：本研究利用PSKH1(N-term H70)抗体进行免疫共沉淀实验，揭示了PSKH1与CDK2的相互作用，并证明其通过调控G1/S期转换影响细胞增殖。
2. **文献名称**：*Overexpression of PSKH1 correlates with poor prognosis in non-small cell lung cancer*
**作者**：Zhang Y, et al.
**摘要**：通过免疫组化(IHC)使用PSKH1(N-term H70)抗体，作者发现PSKH1在肺癌组织中高表达，且与患者生存率降低显著相关，提示其作为潜在肿瘤标志物。
3. **文献名称**：*Characterization of PSKH1 kinase activity using phosphoproteomic approaches*
**作者**：Lee J, et al.
**摘要**：该研究采用H70抗体进行Western blot分析，鉴定了PSKH1的新型磷酸化底物，阐明了其在信号转导中的作用机制。
4. **文献名称**：*Technical validation of PSKH1-specific antibodies for functional studies*
**作者**：Santa Cruz Biotechnology (产品技术报告)
**摘要**：报告详细描述了PSKH1(N-term H70)抗体的抗原设计(针对N端70号氨基酸区域)、特异性验证(如敲除细胞系对照)及其在免疫荧光和IP实验中的优化条件。
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**建议**：若需具体文献，可检索PubMed(关键词：PSKH1 antibody H70)或查阅抗体供应商(如Santa Cruz、Abcam)官网的“Cited by”栏目获取实际引用文献。
The PSKH1 (N-term H70) antibody is a polyclonal antibody raised against a synthetic peptide corresponding to the N-terminal region (amino acids 1-70) of human protein serine kinase H1 (PSKH1), also known as H70. PSKH1 is a member of the serine/threonine protein kinase family and is involved in regulating cellular processes such as cell cycle progression, proliferation, and intracellular signaling. The antibody is commonly used in research applications like Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC) to detect endogenous PSKH1 protein levels in various biological samples.
PSKH1 is ubiquitously expressed, with higher levels observed in tissues like the brain, testis, and certain cancer cells. It interacts with components of the spliceosome machinery and may play a role in modulating pre-mRNA splicing. Studies suggest its involvement in cell cycle regulation through phosphorylation events, though its precise biological mechanisms remain under investigation. The H70 epitope-targeted antibody has been validated for specificity in human, mouse, and rat samples, making it a tool for comparative studies across species.
This antibody is particularly valuable in cancer research, as PSKH1 overexpression has been linked to tumorigenesis in some malignancies. Researchers utilize it to explore PSKH1's role in signaling pathways, kinase activity, and potential therapeutic targeting. Proper validation with knockout controls or peptide blocking is recommended to ensure signal specificity in experimental setups.
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