| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Pantothenate kinase 3, hPanK3, Pantothenic acid kinase 3, PANK3 |
| Entrez GeneID | 79646 |
| WB Predicted band size | 41.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PANK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PANK3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PANK3 (N-term)抗体的参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库查询验证)：
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1. **文献名称**: *"Characterization of PANK3 in Coenzyme A Biosynthesis: Role of N-terminal Domain"*
**作者**: Smith A, et al.
**摘要**: 本研究通过PANK3 (N-term)抗体进行免疫印迹和免疫荧光实验，揭示了PANK3蛋白在肝脏组织中的亚细胞定位，并证明其N端结构域对辅酶A合成的催化活性具有关键作用。
2. **文献名称**: *"Genetic Variants of PANK3 and Their Association with Neurodegenerative Disorders"*
**作者**: Lee J, et al.
**摘要**: 利用PANK3 (N-term)抗体分析患者细胞系中的蛋白表达水平，发现特定突变导致PANK3表达降低，提示其与脑内辅酶A代谢异常及神经退行性病变相关。
3. **文献名称**: *"Antibody-based Profiling of Pantothenate Kinase Isoforms in Human Tissues"*
**作者**: Chen R, et al.
**摘要**: 比较了PANK1-4的抗体特异性，其中PANK3 (N-term)抗体在心脏和肾脏中检测到高丰度表达，表明该异构体在特定组织代谢中的独特功能。
4. **文献名称**: *"Structural Insights into PANK3 Function via N-terminal Epitope Mapping"*
**作者**: Gupta S, et al.
**摘要**: 通过PANK3 (N-term)抗体的表位定位实验，结合X射线晶体学，解析了N端结构域与底物结合的分子机制，为靶向药物设计提供依据。
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建议通过PubMed、Web of Science等平台，使用关键词“PANK3 antibody”或“PANK3 N-terminal”进一步检索真实文献。部分抗体生产商(如Thermo Fisher、Abcam)的产品说明书也可能引用相关文献。
The PANK3 (N-term) antibody is designed to target the N-terminal region of human pantothenate kinase 3 (PANK3), a key enzyme in coenzyme A (CoA) biosynthesis. PANK3 belongs to the pantothenate kinase family (PANK1-4) and catalyzes the first rate-limiting step in CoA synthesis: phosphorylation of pantothenate (vitamin B5) to 4'-phosphopantothenate. CoA is essential for fatty acid metabolism, cellular energy production, and neurotransmitter synthesis. While PANK isoforms share functional similarities, their tissue distribution and regulatory mechanisms differ. PANK3 is predominantly expressed in the liver and brain, with mutations linked to CoA-related metabolic disorders.
This antibody is commonly used in research to study PANK3 expression, localization, and molecular interactions via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Targeting the N-terminal region ensures specificity, as this domain contains unique epitopes absent in other PANK isoforms, aiding in distinguishing PANK3 from homologous proteins like PANK1 or PANK2. Its development supports investigations into PANK3's role in metabolic diseases, neurodegeneration, and potential therapeutic targeting. Validation often includes testing on knockout controls or recombinant proteins to confirm minimal cross-reactivity. Researchers utilize this tool to explore PANK3’s regulatory mechanisms, including feedback inhibition by acetyl-CoA, and its implications in cellular homeostasis.
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