| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Pantothenate kinase 1, hPanK, hPanK1, Pantothenic acid kinase 1, PANK1, PANK |
| Entrez GeneID | 53354 |
| WB Predicted band size | 64.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PANK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 332-360 amino acids from the Central region of human PANK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于PANK1抗体的3篇参考文献摘要整理：
1. **文献名称**: *"Characterization of a Novel Monoclonal Antibody Against Human PANK1 for Detecting Pantothenate Kinase Activity"*
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种针对人源PANK1蛋白的新型单克隆抗体，验证了其在Western blot和免疫组化中的特异性。该抗体成功用于检测细胞模型中PANK1的表达水平，并揭示其在泛酸代谢通路中的功能调控。
2. **文献名称**: *"PANK1 Dysregulation in Neurodegenerative Disorders: Insights from Antibody-Based Profiling"*
**作者**: Lee JH, Kim S.
**摘要**: 通过使用商业化PANK1抗体，研究者分析了阿尔茨海默病和帕金森病患者脑组织样本中的PANK1表达模式。结果显示PANK1蛋白水平显著降低，提示其与神经退行性病变的能量代谢异常相关。
3. **文献名称**: *"Antibody Validation and Structural Analysis of Pantothenate Kinase 1 (PANK1) in Coenzyme A Biosynthesis"*
**作者**: Garcia R, et al.
**摘要**: 该文献系统验证了多种PANK1抗体的抗原表位及交叉反应性，结合X射线晶体学数据解析了PANK1的催化结构域。研究强调了选择高特异性抗体对准确研究酶活性及药物靶点筛选的重要性。
注：上述文献信息为示例性内容，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。若需具体文献，建议使用关键词“PANK1 antibody”或“pantothenate kinase 1 immunohistochemistry”进一步查询。
The PANK1 antibody is a tool used to detect and study pantothenate kinase 1 (PANK1), an enzyme critical for coenzyme A (CoA) biosynthesis. CoA is essential for cellular metabolism, including fatty acid synthesis, energy production, and detoxification. PANK1 catalyzes the first rate-limiting step in the CoA pathway by phosphorylating pantothenate (vitamin B5). Unlike other isoforms (PANK2. PANK3. PANK4), PANK1 exists in two splice variants (PANK1α and PANK1β) with distinct subcellular localizations and regulatory roles. Dysregulation of PANK1 is linked to metabolic disorders, neurodegeneration, and cancer, making it a focus of biomedical research.
PANK1 antibodies are widely utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze protein expression, tissue distribution, and subcellular localization. They help investigate PANK1’s role in cellular responses to metabolic stress, nutrient sensing, and disease mechanisms. For instance, studies have explored PANK1’s association with insulin resistance, hepatic steatosis, and neuronal survival. Commercial PANK1 antibodies are typically raised in rabbits or mice against specific epitopes, with validation in knockout models to ensure specificity. However, cross-reactivity with other PANK isoforms remains a potential concern, requiring careful experimental controls. These antibodies contribute to advancing understanding of CoA-dependent pathways and therapeutic targeting of metabolic diseases.
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