| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cysteine protease ATG4D, 3422-, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D, mitochondrial, ATG4D, APG4D, AUTL4 |
| Entrez GeneID | 84971 |
| WB Predicted band size | 52.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ATG4D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the Central region of human ATG4D. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于ATG4D抗体的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库查询确认)：
1. **文献名称**：*ATG4D regulates neuronal mitophagy and suppresses neuroinflammation in Parkinson's disease models*
**作者**：Li J, et al.
**摘要**：本研究利用特异性ATG4D抗体证实了其在神经元线粒体自噬中的核心作用，发现ATG4D缺失会加剧帕金森模型中的炎症反应，抗体检测显示其表达水平与疾病进展负相关。
2. **文献名称**：*Development of a monoclonal antibody targeting the catalytic domain of human ATG4D for autophagy inhibition*
**作者**：Chen X, et al.
**摘要**：文章报道了一种靶向ATG4D催化结构域的单克隆抗体的开发，该抗体能有效抑制其蛋白酶活性，并通过Western blot和免疫荧光验证了其在癌细胞自噬调控中的应用潜力。
3. **文献名称**：*ATG4D as a novel prognostic biomarker in colorectal cancer: Insights from antibody-based tissue microarray analysis*
**作者**：Wang Y, et al.
**摘要**：通过组织芯片免疫组化分析，研究发现ATG4D在结直肠癌中高表达且与患者生存率下降相关，提示其可能作为预后标志物，研究使用商业化ATG4D抗体进行大规模样本验证。
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**提示**：实际文献建议通过PubMed/Google Scholar以"ATG4D antibody"或"ATG4D autophagy"为关键词检索，并优先选择近5年、高被引研究。部分研究可能侧重ATG4家族共性而非单独讨论ATG4D，需注意筛选。
ATG4D, a member of the ATG4 cysteine protease family, plays a critical role in autophagy—a cellular recycling process that degrades damaged organelles and proteins. Specifically, ATG4D cleaves pro-LC3 (microtubule-associated protein 1 light chain 3) to generate its cytosolic form, enabling its conjugation to autophagosomal membranes during autophagosome formation. Unlike other ATG4 isoforms (A, B, C), ATG4D exhibits distinct regulatory roles, including potential involvement in selective autophagy under stress conditions like endoplasmic reticulum stress or proteasome inhibition. Its activity is tightly modulated by post-translational modifications and interactions with other autophagy-related proteins.
ATG4D antibodies are essential tools for studying its expression, localization, and function in autophagy. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore ATG4D’s contribution to cellular homeostasis, disease mechanisms (e.g., cancer, neurodegeneration), and its crosstalk with pathways like apoptosis. Research also focuses on ATG4D’s potential as a therapeutic target, as autophagy dysregulation is linked to numerous pathologies. Validated antibodies help distinguish ATG4D from other isoforms, aiding in isoform-specific mechanistic studies. However, challenges remain in ensuring antibody specificity due to high sequence homology within the ATG4 family.
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