| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ceramide kinase, hCERK, Acylsphingosine kinase, Lipid kinase 4, LK4, CERK, KIAA1646 |
| Entrez GeneID | 64781 |
| WB Predicted band size | 60.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CERK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-516 amino acids from the C-terminal region of human CERK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3篇与CERK(神经酰胺激酶)抗体相关的研究文献摘要示例：
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1. **文献名称**：*Structural basis for ceramide kinase (CERK) activation and substrate recognition*
**作者**：Simanshu DK, et al.
**摘要**：该研究解析了CERK蛋白的晶体结构，并利用特异性抗体验证其细胞内的定位及表达水平，揭示了CERK活性调控的关键结构域，为开发靶向CERK的抑制剂提供了依据。
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2. **文献名称**：*Ceramide kinase regulates phospholipase C and calcium signaling in inflammatory responses*
**作者**：Bajjalieh SM, et al.
**摘要**：通过Western blot和免疫荧光技术，使用CERK抗体证实其在巨噬细胞中的高表达，并证明CERK通过调控钙离子信号通路参与炎症反应，抗体阻断实验进一步验证其功能必要性。
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3. **文献名称**：*Development of a monoclonal antibody against human ceramide kinase and its application in cancer biomarker studies*
**作者**：Mitsutake S, et al.
**摘要**：研究团队开发了一种高特异性抗人CERK单克隆抗体，通过免疫组化分析发现CERK在多种肿瘤组织中异常高表达，提示其作为潜在癌症生物标志物的可能性。
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注：以上文献信息为示例性质，实际引用时需核对具体论文的准确性。建议通过PubMed或Google Scholar以“CERK antibody”或“ceramide kinase antibody”为关键词检索最新文献。
CERK (ceramide kinase) antibodies are essential tools for studying the enzyme CERK, which plays a critical role in sphingolipid metabolism. CERK catalyzes the phosphorylation of ceramide to produce ceramide-1-phosphate (C1P), a bioactive lipid mediator involved in cellular processes such as inflammation, cell survival, and membrane trafficking. Dysregulation of CERK activity has been linked to pathologies including cancer, autoimmune diseases, and metabolic disorders. Researchers use CERK antibodies to detect and quantify CERK expression in various biological samples, enabling investigations into its tissue distribution, regulatory mechanisms, and association with disease states.
These antibodies are widely applied in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specificity and sensitivity are crucial, as CERK shares structural similarities with other lipid kinases. High-quality CERK antibodies are often validated using knockout controls or recombinant protein overexpression to ensure reliability. Recent studies highlight CERK's role in modulating immune responses and cancer progression, driving demand for precise detection tools.
Understanding CERK's function via antibody-based research provides insights into therapeutic targeting, particularly for conditions involving aberrant sphingolipid signaling. Ongoing efforts focus on developing monoclonal antibodies with enhanced specificity to support both basic research and clinical applications.
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