| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ethanolamine kinase 1, EKI 1, ETNK1, EKI1 |
| Entrez GeneID | 55500 |
| WB Predicted band size | 51.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Ethanolamine Kinase (EKI1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Ethanolamine Kinase (EKI1). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Ethanolamine Kinase (EKI1) (N-term)抗体的假设参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Characterization of Ethanolamine Kinase 1 (EKI1) in Plant Phospholipid Metabolism"*
**作者**: Müller et al. (2008)
**摘要**: 本研究利用针对EKI1 N端的多克隆抗体，通过Western blot和免疫荧光技术，揭示了EKI1在拟南芥根尖细胞中的亚细胞定位，并证实其在乙醇胺磷酸化途径中的关键作用。抗体特异性通过基因敲除突变体验证。
2. **文献名称**: *"Antibody-Based Profiling of Ethanolamine Kinase Isoforms in Mammalian Tissues"*
**作者**: Lee & Kim (2012)
**摘要**: 作者开发了一种靶向EKI1 N端表位的单克隆抗体，用于比较不同哺乳动物组织中EKI1的表达水平。研究发现肝脏和脑组织中EKI1丰度最高，且抗体在免疫沉淀实验中表现出高特异性。
3. **文献名称**: *"Functional Analysis of EKI1 in Yeast Phospholipid Biosynthesis"*
**作者**: Costa et al. (2015)
**摘要**: 通过N端特异性抗体抑制实验，证明酿酒酵母中EKI1的酶活性缺失会导致磷脂酰乙醇胺合成受阻。抗体被用于蛋白互作组学分析，揭示EKI1与细胞膜脂质转运蛋白的相互作用。
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**注意**：以上为示例文献，实际研究中请通过PubMed、Google Scholar等平台检索真实文献(关键词：EKI1 antibody, ethanolamine kinase antibody, N-terminal epitope)。如需具体文献协助，可提供更详细的研究背景。
Ethanolamine Kinase (EKI1) (N-term) antibody is a targeted immunological tool designed to recognize the N-terminal region of the ethanolamine kinase 1 enzyme, encoded by the EKI1 gene in humans. Ethanolamine kinases, including EKI1. belong to the choline/ethanolamine kinase family and play a critical role in phospholipid metabolism. Specifically, EKI1 catalyzes the ATP-dependent phosphorylation of ethanolamine to produce phosphoethanolamine, a key precursor for the synthesis of phosphatidylethanolamine (PE)—a major phospholipid in cellular membranes. This enzyme is ubiquitously expressed, with notable activity in the liver, brain, and other tissues requiring robust membrane synthesis or repair.
The antibody against the N-terminal region of EKI1 is commonly utilized in research to study the enzyme’s expression, localization, and regulatory mechanisms. It is employed in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to investigate EKI1’s role in cellular processes, including lipid homeostasis, cell signaling, and membrane dynamics. Studies have linked EKI1 dysfunction to metabolic disorders, neurodegenerative diseases (e.g., Alzheimer’s), and cancer, highlighting its biological relevance. As a polyclonal or monoclonal antibody, it is often validated for specificity using knockout controls or siRNA-mediated silencing. Researchers rely on this tool to explore EKI1’s interactions within the Kennedy pathway and its potential as a therapeutic target in lipid-related pathologies.
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