| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hsc70-interacting protein, Hip, Aging-associated protein 2, Progesterone receptor-associated p48 protein, Protein FAM10A1, Putative tumor suppressor ST13, Renal carcinoma antigen NY-REN-33, Suppression of tumorigenicity 13 protein, ST13, AAG2, FAM10A1, HIP, SNC6 |
| Entrez GeneID | 6767 |
| WB Predicted band size | 41.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This HSC70 Interacting Protein HIP (ST13) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-80 amino acids from human HSC70 Interacting Protein HIP (ST13). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HSC70 Interacting Protein HIP (ST13)抗体的参考文献示例，按文献名称、作者和摘要内容概括整理：
1. **"ST13 regulates colorectal cancer cell proliferation and apoptosis via the Hippo signaling pathway"**
- **作者**: Zhang, Y., et al.
- **摘要**: 该研究利用ST13抗体进行免疫组化和Western blot分析，发现ST13通过调控Hippo信号通路抑制结直肠癌细胞增殖并促进凋亡，其低表达与患者不良预后相关。
2. **"Hsp70/Hsc70 system cooperates with co-chaperones in protein folding and disease"**
- **作者**: Hohfeld, J., & Jentsch, S.
- **摘要**: 这篇综述探讨了HSC70与辅助蛋白(如HIP)在蛋白质折叠中的协同机制，指出ST13抗体在解析HSC70-HIP复合物结构及功能研究中的关键作用。
3. **"The role of co-chaperones in modulating Hsp70/Hsc70 function in cellular stress"**
- **作者**: Kampinga, H.H., et al.
- **摘要**: 研究通过免疫共沉淀结合ST13抗体，揭示HIP在应激条件下调控HSC70的ATP酶活性，影响错误折叠蛋白的清除效率，尤其在神经退行性疾病模型中表现显著。
4. **"ST13/HIP as a potential biomarker in cancer progression"**
- **作者**: Daugaard, M., et al.
- **摘要**: 该文献利用ST13抗体检测多种肿瘤组织样本，发现HIP在乳腺癌和肺癌中高表达，并通过调控HSC70介导的客户蛋白稳定性促进肿瘤转移。
注：上述文献为示例性质，具体内容需根据实际发表研究调整。建议通过PubMed或Web of Science以关键词“ST13 antibody”或“HIP HSC70”检索最新文献。
The HSC70 Interacting Protein (HIP), encoded by the *ST13* gene, is a co-chaperone that regulates the Hsc70/Hsp70 molecular chaperone system. HIP binds directly to the ATPase domain of Hsc70. stabilizing its ADP-bound state and facilitating substrate transfer to downstream chaperones like Hsp90. This interaction is critical for protein folding, trafficking, and degradation processes, particularly under cellular stress. HIP also plays a role in modulating apoptosis, autophagy, and steroid hormone receptor signaling.
Antibodies targeting HIP (ST13) are essential tools for studying its expression, localization, and interactions in various biological contexts. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to explore HIP's involvement in diseases like cancer and neurodegenerative disorders. For example, HIP has been implicated in cancer progression, where it may act as a tumor suppressor by promoting apoptosis, though conflicting studies suggest context-dependent roles in metastasis. In neurodegenerative diseases, HIP's interaction with Hsp70 is linked to the clearance of misfolded proteins, such as amyloid-beta in Alzheimer's disease.
Validated HIP antibodies are crucial for distinguishing HIP from other chaperone components and assessing its regulation under stress conditions (e.g., heat shock). Researchers often verify specificity using knockout cell lines or siRNA knockdown. Reliable antibodies enable insights into HIP's dual roles in proteostasis and disease mechanisms, making them vital for both basic and translational research.
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