| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein ENL, YEATS domain-containing protein 1, MLLT1, ENL, LTG19, YEATS1 |
| Entrez GeneID | 4298 |
| WB Predicted band size | 62.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MLLT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 523-552 amino acids from the C-terminal region of human MLLT1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MLLT1抗体的3篇示例文献(注：以下为模拟内容，实际引用时请核实真实文献)：
1. **文献名称**：The role of MLLT1 in the Super Elongation Complex regulates transcriptional control
**作者**：Shilatifard A, et al.
**摘要**：研究揭示了MLLT1作为超级延伸复合物(SEC)的关键组分，通过染色质免疫沉淀(ChIP)和Western blot实验(使用MLLT1特异性抗体)，证实其与RNA聚合酶II的相互作用，调控白血病相关基因的转录延伸。
2. **文献名称**：MLLT1 fusion partners in MLL-rearranged leukemias: functional characterization
**作者**：Meyer C, et al.
**摘要**：通过免疫共沉淀和免疫荧光技术(基于抗MLLT1抗体)，分析了MLLT1与MLL蛋白的融合机制，发现其在白血病细胞中异常招募组蛋白修饰酶，驱动致癌基因表达。
3. **文献名称**：CRISPR screening identifies MLLT1 as a dependency in acute myeloid leukemia
**作者**：Smith J, Doe R.
**摘要**：利用CRISPR-Cas9筛选结合MLLT1抗体(Western blot验证敲除效率)，发现MLLT1缺失抑制白血病细胞增殖，并降低HOX基因表达，提示其作为治疗靶点的潜力。
4. **文献名称**：Structural insights into MLLT1’s YEATS domain and histone recognition
**作者**：Chen Z, et al.
**摘要**：通过X射线晶体学和免疫印迹(使用MLLT1抗体验证蛋白纯化)，解析了MLLT1的YEATS结构域识别组蛋白乙酰化修饰的分子机制，为其在表观遗传调控中的作用提供依据。
(提示：以上文献信息为示例，实际研究中请通过PubMed或Google Scholar检索真实文献。)
**Background of MLLT1 Antibody**
The MLLT1 (Mixed-Lineage Leukemia Translocated to 1) gene, also known as ENL or YEATS1. encodes a chromatin-associated protein involved in transcriptional regulation and epigenetic modifications. It is a critical component of the super elongation complex (SEC) and plays a role in RNA polymerase II-mediated transcription elongation. MLLT1 contains a conserved YEATS domain, which binds acetylated or crotonylated histone marks, linking chromatin modifications to transcriptional activation.
MLLT1 gained prominence due to its involvement in chromosomal translocations with the MLL (KMT2A) gene in acute leukemias, particularly in infants. The resulting MLL-MLLT1 fusion protein disrupts normal epigenetic regulation, driving aberrant expression of oncogenic genes like HOX clusters. This fusion is associated with aggressive leukemia subtypes, underscoring MLLT1's role in oncogenesis.
MLLT1 antibodies are essential tools for studying its expression, localization, and interactions in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate MLLT1's function in transcriptional regulation, chromatin remodeling, and leukemia biology. These antibodies also aid in diagnosing MLL-rearranged leukemias and evaluating therapeutic strategies targeting MLLT1-containing complexes.
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