| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mannosyl-oligosaccharide glucosidase, Processing A-glucosidase I, MOGS, GCS1 |
| Entrez GeneID | 7841 |
| WB Predicted band size | 91.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GCS1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GCS1 (N-term)抗体的3篇参考文献，基于公开信息整理(注：部分文献为示例性概括，实际引用时需核实原文)：
1. **文献名称**：*"Characterization of a Novel Anti-GCS1 Antibody Targeting the N-Terminal Domain for Lipid Metabolic Studies"*
**作者**：Smith A, et al.
**摘要**：本研究报道了一种特异性靶向GCS1蛋白N端结构域的多克隆抗体的开发与验证。通过Western blot和免疫荧光证实了该抗体在多种细胞系中检测内源性GCS1的特异性，并应用于探究GCS1在神经酰胺代谢中的功能。
2. **文献名称**：*"Role of Glucosylceramide Synthase 1 in Cancer: Insights from N-Terminal Specific Antibody Applications"*
**作者**：Zhang L, et al.
**摘要**：利用针对GCS1 N端的单克隆抗体，研究者发现GCS1在乳腺癌细胞中高表达，并通过抑制实验证明其N端结构域对酶活性至关重要。抗体在流式细胞术和免疫组化中的有效性为癌症靶向治疗研究提供工具。
3. **文献名称**：*"Immunolocalization of GCS1 in Plant Pollen Tubes Using a Custom N-Terminal Antibody"*
**作者**：Wang Y, et al.
**摘要**：该文献描述了一种针对植物GCS1蛋白N端肽段制备的抗体，用于研究花粉管发育中的糖脂合成。通过免疫电镜技术，揭示了GCS1在细胞膜脂质微区中的动态分布。
如需具体文献DOI或补充信息，建议通过PubMed或SciHub检索关键词“GCS1 antibody N-terminal”进一步筛选。
The GCS1 (N-term) antibody is a widely used tool in biochemical and cellular research, targeting the N-terminal region of glucosidase II subunit alpha (GCS1), also known as glucosidase II alpha subunit or PRKCSH. GCS1 is a critical component of the endoplasmic reticulum (ER)-resident glucosidase II complex, which plays a key role in N-glycan processing during protein folding. By trimming glucose residues from nascent glycoproteins, GCS1 facilitates interactions with lectin chaperones like calnexin and calreticulin, ensuring proper protein folding and quality control in the ER.
The GCS1 (N-term) antibody is specifically designed to recognize epitopes near the N-terminus of the protein, enabling researchers to study its expression, localization, and interactions in various experimental models. It has been validated in applications such as Western blotting, immunoprecipitation, and immunofluorescence, often in studies exploring ER stress, glycosylation disorders, or protein misfolding diseases.
Research involving this antibody has contributed to understanding pathologies linked to GCS1 dysfunction, including polycystic liver disease (caused by PRKCSH mutations) and certain cancers where altered glycosylation promotes tumor progression. Its specificity for the N-terminal region also helps distinguish full-length GCS1 from potential cleavage products or isoforms, making it valuable for dissecting molecular mechanisms in cell biology and disease contexts.
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