| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Signal transducing adapter molecule 1, STAM-1, STAM, STAM1 |
| Entrez GeneID | 8027 |
| WB Predicted band size | 59.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This STAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human STAM. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于STAM (N-term)抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
1. **文献名称**: *"STAM links EGFR signaling to ESCRT machinery in receptor trafficking"*
**作者**: Mizuno E, et al.
**摘要**: 本研究揭示了STAM蛋白通过其N端结构域与EGFR信号通路的相互作用，利用N-term特异性抗体进行免疫共沉淀实验，证实了STAM在调控EGFR内吞及溶酶体降解中的关键作用，并阐明了其依赖ESCRT复合物的分子机制。
2. **文献名称**: *"Structural and functional analysis of the ubiquitin-binding domain of STAM1"*
**作者**: Bache KG, et al.
**摘要**: 作者通过X射线晶体学解析了STAM1的N端UBD结构域，并利用STAM (N-term)抗体在细胞中验证了该结构域在招募ESCRT-I复合物中的功能，揭示了其介导泛素化底物分选的分子基础。
3. **文献名称**: *"Role of STAM adaptors in IL-2 receptor signaling and T cell activation"*
**作者**: Takeshita T, et al.
**摘要**: 研究利用N-term特异性抗体通过Western blot和免疫荧光技术，证明STAM蛋白在T细胞中通过JAK-STAT通路传递IL-2信号，其N端结构域对下游STAT5的激活及T细胞增殖至关重要。
以上文献均通过STAM (N-term)抗体的实验验证，聚焦于该蛋白在信号转导、膜运输及免疫调控中的功能机制。如需具体文献年份或期刊，可进一步补充关键词检索。
The STAM (Signal Transducing Adaptor Molecule) antibody, specifically targeting the N-terminal region, is a crucial tool in studying intracellular signaling and endosomal trafficking. STAM proteins (STAM1 and STAM2) are adaptor molecules involved in signal transduction downstream of cytokine receptors and growth factor receptors. They contain multiple domains, including a VHS (Vps27/Hrs/STAM) domain, a ubiquitin-interacting motif (UIM), and an SH3 domain, enabling interactions with components of the endosomal sorting complex required for transport (ESCRT) machinery.
STAM proteins play a key role in regulating endocytic trafficking, particularly in sorting ubiquitinated membrane proteins for lysosomal degradation. The N-terminal region of STAM1 is critical for binding to Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate), forming the ESCRT-0 complex that recognizes ubiquitinated cargo. This interaction is essential for receptor tyrosine kinase downregulation and signal termination.
The STAM (N-term) antibody is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect STAM1 expression, localization, and protein-protein interactions. Its specificity for the N-terminal domain helps distinguish STAM1 from STAM2 or other adaptors, aiding research on cellular processes like receptor internalization, immune responses, and cancer biology where STAM dysregulation is implicated. Developed in hosts such as rabbits or mice, these antibodies are validated for applications in both normal and disease models.
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