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Rabbit Polyclonal RBCK1(UBCE7IP3)(N-term) Antibody

  • 中文名： RBCK1 (UBCE7IP3) (N-term)抗体
  • 别    名： RanBP-type and C3HC4-type zinc finger-containing protein 1, 632-, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, HOIL-1, Hepatitis B virus X-associated protein 4, RING finger protein 54, Ubiquitin-conjugating enzyme 7-interacting protein
货号: IPDX30061
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRanBP-type and C3HC4-type zinc finger-containing protein 1, 632-, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, HOIL-1, Hepatitis B virus X-associated protein 4, RING finger protein 54, Ubiquitin-conjugating enzyme 7-interacting protein 3, RBCK1, C20orf18, RNF54, UBCE7IP3, XAP3, XAP4
Entrez GeneID10616
WB Predicted band size57.6kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis RBCK1 (UBCE7IP3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human RBCK1 (UBCE7IP3).
FormulationPurified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) .

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参考文献

以下是关于 **RBCK1 (UBCE7IP3) N-term抗体** 的3篇参考文献及其摘要内容：

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1. **文献名称**: *RBCK1. an E3 ubiquitin ligase, interacts with parkin and promotes its aggresome formation*

**作者**: Tanaka K, Suzuki T, Chiba T

**摘要**: 研究揭示RBCK1作为HOIL-1L泛素连接酶复合物的成员，通过其N端结构域与帕金蛋白(parkin)相互作用，参与异常蛋白聚集体的泛素化及自噬清除。实验中使用了针对RBCK1 N端的抗体验证其亚细胞定位。

2. **文献名称**: *The linear ubiquitin chain assembly complex (LUBAC) regulates NF-κB signaling and inflammation*

**作者**: Tokunaga F, Iwai K

**摘要**: 本文阐明了RBCK1(HOIL-1L)与SHARPIN、HOIP组成LUBAC复合体，通过N端结构域介导线性泛素链合成，调控NF-κB信号通路。研究中利用RBCK1 N-term抗体检测其在炎症反应中的表达变化。

3. **文献名称**: *RBCK1 deficiency leads to autoimmune disorders via impaired linear ubiquitination*

**作者**: Boisson B, Laplantine E, Dobbs K, et al.

**摘要**: 通过分析RBCK1基因缺陷患者，发现其N端结构域对LUBAC复合体稳定性至关重要，缺陷导致线性泛素化异常及免疫失调。抗体实验证实RBCK1 N端缺失引发蛋白功能丧失。

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以上文献均涉及RBCK1的N端功能研究，并使用了特异性抗体验证其分子机制。如需具体实验细节，建议查阅全文。

背景信息

The RBCK1 (UBCE7IP3) (N-term) antibody is designed to target the N-terminal region of the RBCK1 protein, also known as UBE2J3-interacting protein 3 or HOIL-1. RBCK1 is a critical component of the linear ubiquitin chain assembly complex (LUBAC), a multisubunit E3 ubiquitin ligase that regulates NF-κB signaling by catalyzing the formation of linear polyubiquitin chains on substrate proteins. This post-translational modification is essential for immune response, inflammation, and cell death pathways. The N-terminal region of RBCK1 contains a ubiquitin-like (UBL) domain and a RanBP2-type zinc finger motif, which facilitate protein-protein interactions and complex assembly. Antibodies specific to this region are widely used in studies investigating LUBAC function, NF-κB activation mechanisms, and dysregulation in diseases such as cancer, autoinflammatory disorders, and immune deficiencies. Mutations in RBCK1 have been linked to polyglucosan body myopathy type 2 (PGBM2), a rare genetic disorder characterized by progressive muscle weakness. Researchers employ this antibody in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect RBCK1 expression, assess its interaction partners, or evaluate its role in ubiquitination-dependent signaling. Its specificity for the N-terminal epitope ensures minimal cross-reactivity with other LUBAC components, aiding precise mechanistic studies.

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