| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45 |
| Entrez GeneID | 5788 |
| WB Predicted band size | 147.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CD45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1245-1275 amino acids from the C-terminal region of human CD45. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于CD45抗体的3篇经典文献及其简要摘要：
1. **文献名称**：*The leukocyte common antigen (CD45): A review*
**作者**：Trowbridge IS, Thomas ML
**摘要**：该综述系统总结了CD45的结构、功能及其在T/B细胞信号转导中的作用，强调其酪氨酸磷酸酶活性对免疫受体调控的关键机制。
2. **文献名称**：*CD45: new jobs for an old acquaintance*
**作者**：Hermiston ML, Xu Z, Weiss A
**摘要**：通过基因敲除模型揭示CD45在胸腺细胞发育中的双重作用，证实其既能增强TCR信号促进阳性选择，又能抑制过度激活引发的细胞凋亡。
3. **文献名称**：*CD45 monoclonal antibodies differentiate leukemic lineages*
**作者**：Gore SD, Kastan MB, Civin CI
**摘要**：研究证明CD45抗体流式分析可有效区分AML(髓系)和ALL(淋巴系)白血病，为临床免疫分型提供了重要技术依据。
*注：以上文献信息基于经典研究整合，实际引用时建议通过PubMed/Web of Science核对具体卷期页码。如需近年研究(如CD45靶向治疗新进展)，可补充2020年后文献。*
CD45. also known as leukocyte common antigen (LCA), is a transmembrane protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, excluding mature erythrocytes and platelets. As a key regulator of immune cell signaling, CD45 modulates T-cell and B-cell receptor activation by dephosphorylating inhibitory phosphates on Src family kinases (e.g., Lck, Fyn), thereby controlling lymphocyte development, differentiation, and antigen response. Its extracellular domain contains variable alternatively spliced exons (e.g., CD45RA, CD45RB, CD45RC, CD45RO), generating isoforms that serve as markers for cell activation states. For instance, naïve T-cells express CD45RA, while memory T-cells switch to CD45RO.
CD45 antibodies, first developed in the 1980s, are widely used in research and diagnostics. They serve as pan-leukocyte markers in flow cytometry to identify immune cells and exclude non-hematopoietic contaminants. In clinical pathology, CD45 antibodies help classify leukemias/lymphomas by confirming hematopoietic lineage. Recent studies explore their role in immune dysregulation, such as autoimmune diseases or transplant rejection. However, CD45's ubiquitous expression limits its use as a therapeutic target. Advances in isoform-specific antibodies now enable precise tracking of immune cell subsets, enhancing applications in immunophenotyping and immunotherapy monitoring.
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