| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Neuronal calcium sensor 1, NCS-1, Frequenin homolog, Frequenin-like protein, Frequenin-like ubiquitous protein, NCS1, FLUP, FREQ |
| Entrez GeneID | 23413 |
| WB Predicted band size | 21.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-144 amino acids from the Central region of human NCS1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NCS1抗体的3-4篇参考文献示例(文献信息为模拟，仅供参考)：
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1. **文献名称**: "Development of a Specific Antibody for Neuronal Calcium Sensor 1 (NCS1) and Its Application in Neurodegenerative Disease Models"
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种兔源多克隆抗体，针对NCS1蛋白的C末端区域。通过Western blot和免疫组化验证其特异性，发现其在帕金森病模型中可有效检测NCS1的表达变化，为神经退行性疾病研究提供工具。
2. **文献名称**: "Characterization of Commercial NCS1 Antibodies: Implications for Calcium Signaling Studies"
**作者**: Zhang L, et al.
**摘要**: 对比分析了多种市售NCS1抗体的特异性，发现部分抗体存在与类似钙传感器蛋白(如NCS家族其他成员)的交叉反应。研究强调抗体验证在神经科学研究中的重要性。
3. **文献名称**: "NCS1 Antibody-Based Profiling Reveals Altered Expression in Schizophrenia Postmortem Brains"
**作者**: Tanaka K, et al.
**摘要**: 利用高特异性NCS1抗体对精神分裂症患者脑组织进行检测，发现前额叶皮层中NCS1蛋白水平显著降低，提示其可能与精神疾病病理机制相关。
4. **文献名称**: "A Monoclonal Antibody Targeting NCS1 Disrupts Its Interaction with Dopamine Receptors"
**作者**: Rodriguez M, et al.
**摘要**: 开发了一种单克隆抗体，可阻断NCS1与多巴胺受体D2的相互作用，并通过体外实验证明该抗体能够调节下游信号通路，为靶向NCS1的药物研发提供新策略。
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**注**：以上文献为示例，实际引用时请通过PubMed、Google Scholar等平台核实真实文献信息及摘要内容。
The Neuronal Calcium Sensor 1 (NCS1) antibody is a critical tool for studying the NCS1 protein, a member of the EF-hand calcium-binding protein family. NCS1. also known as frequenin in invertebrates, plays a vital role in calcium-dependent signaling pathways, particularly in neuronal and endocrine systems. It regulates diverse cellular processes, including synaptic plasticity, neurotransmitter release, ion channel activity, and cell survival. Dysregulation of NCS1 has been implicated in neurological disorders (e.g., Alzheimer’s disease, schizophrenia, bipolar disorder) and cardiovascular conditions, making it a focus of biomedical research.
NCS1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect NCS1 expression, localization, and interactions in tissues or cultured cells. These antibodies are typically raised in rabbits or mice against specific epitopes of the human or rodent NCS1 protein. Their specificity is often validated via knockout controls or siRNA silencing. Due to NCS1’s structural homology with other calcium sensors (e.g., recoverin, neurocalcin), antibody validation is crucial to avoid cross-reactivity.
Research using NCS1 antibodies has revealed its dual roles in health and disease, such as promoting neuronal survival at physiological levels while contributing to pathological calcium overload in neurodegeneration. Additionally, NCS1's interaction with dopamine receptors and GPCRs highlights its therapeutic potential. However, variability in antibody performance across commercial sources underscores the need for rigorous experimental optimization.
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