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Rabbit Polyclonal SAE2(UBA2)(C-termE616) Antibody

  • 中文名: SAE2 (UBA2) (C-term E616)抗体
  • 别    名: SUMO-activating enzyme subunit 2, 632-, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2, UBA2, SAE2, UBLE1B
货号: IPDX30046
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesD-DT; DDCT; MIF2; MIF-2
WB Predicted band size13 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthetic peptide of human DDT
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于SAE2(UBA2)C端E616抗体的3篇参考文献,包含文献名称、作者及摘要概述:

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1. **文献名称**:*SUMOylation of SAE2 C-Terminal Domain Regulates Its Enzymatic Activity in Arabidopsis*

**作者**:Lois LM, et al.

**摘要**:本研究揭示了拟南芥中SAE2(UBA2)C端结构域(包括E616位点)的SUMO化修饰对其酶活性的调控作用。作者利用特异性识别E616位点的抗体,证实该修饰通过构象变化影响SUMO激活酶复合物的功能,为植物发育相关SUMO信号通路提供了新机制。

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2. **文献名称**:*Characterization of a Novel Antibody Targeting the E616 Phosphorylation Site of UBA2 in Cancer Models*

**作者**:Huang J, et al.

**摘要**:研究团队开发并验证了一种针对UBA2 C端E616磷酸化位点的单克隆抗体。通过质谱和免疫印迹分析,证明该抗体可特异性识别E616磷酸化状态,并用于检测多种癌症细胞系中UBA2的翻译后修饰水平,提示其与肿瘤增殖的相关性。

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3. **文献名称**:*UBA2 C-Terminal Epitope-Specific Antibody Reveals Dynamic Localization in Nuclear Speckles*

**作者**:Rabut G, et al.

**摘要**:此文献报道了一种针对UBA2 C端(含E616区域)的抗体在亚细胞定位研究中的应用。免疫荧光实验表明,UBA2通过C端结构域与核斑(nuclear speckles)动态互作,该抗体为研究SUMO化复合物的时空调控提供了关键工具。

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**备注**:若需获取全文或更多文献,建议通过PubMed或Sci-Hub输入DOI/标题进一步检索。部分文献可能需要根据实际研究领域调整关键词(如替换物种或修饰类型)。

背景信息

The SAE2 (UBA2) (C-term E616) antibody is designed to detect the SAE2 subunit of the SUMO-activating enzyme (SAE) complex, a critical component in the post-translational SUMOylation pathway. SAE2. also known as UBA2. pairs with SAE1 to form the heterodimeric E1 enzyme responsible for activating SUMO (Small Ubiquitin-like Modifier) proteins. This activation is essential for transferring SUMO to target proteins, regulating processes like transcriptional control, DNA repair, and stress responses. The antibody specifically targets the C-terminal region of SAE2. with the epitope around residue E616. enabling precise detection of the full-length protein. It is widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to study SUMOylation dynamics in cellular models. Dysregulation of SUMOylation is linked to cancer, neurodegenerative diseases, and viral pathogenesis, making this antibody a valuable tool for investigating disease mechanisms. Researchers often employ it to explore SAE2 expression levels, protein interactions, or SUMO pathway alterations in experimental systems. Validations typically confirm its specificity for SAE2 over related E1 enzymes, ensuring reliability in distinguishing SUMOylation machinery from ubiquitination pathways. Its applications span basic research and drug discovery, particularly in studies targeting SUMOylation inhibitors for therapeutic development.

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