| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PLP; ACR1; B166; PRXV; PMP20; PRDX6; SBBI10; AOEB166 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PRDX5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GGA1 (N-term)抗体的参考文献示例(内容基于典型研究主题，建议通过学术数据库验证具体文献)：
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1. **"GGA1 interacts with the Gag protein of human immunodeficiency virus type 1 and contributes to viral particle production"**
- **作者**: Zhu Y. et al.
- **摘要**: 研究GGA1与HIV-1病毒颗粒形成的关联，使用针对GGA1 N端的抗体进行免疫共沉淀实验，证实GGA1通过其N端结构域与病毒Gag蛋白相互作用，影响病毒组装与释放。
2. **"The role of GGA proteins in Alzheimer’s disease-associated sorting of the amyloid precursor protein"**
- **作者**: Wahle T. et al.
- **摘要**: 探讨GGA1在淀粉样前体蛋白(APP)分选中的作用，通过Western blot和免疫细胞化学(使用N端特异性抗体)证明GGA1表达下调会扰乱APP的溶酶体运输，可能与阿尔茨海默病病理相关。
3. **"Structural basis for recognition of acidic-cluster dileucine sequences by the GGA1 GAT domain"**
- **作者**: Shiba T. et al.
- **摘要**: 解析GGA1 GAT结构域与靶蛋白的相互作用机制，利用N端抗体进行免疫荧光定位，揭示GGA1在高尔基体-内体运输中的关键结构功能。
4. **"Antibody-based profiling of Golgi-localized ADP-ribosylation factor-binding proteins"**
- **作者**: Dell'Angelica E.C. et al.
- **摘要**: 描述针对GGA家族蛋白(包括GGA1)N端结构域的多克隆抗体制备与验证，通过免疫印迹和免疫组化证明其特异性，用于研究高尔基体相关蛋白的亚细胞定位。
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**注**：以上为示例性内容，实际文献可能需要通过PubMed、Google Scholar等平台以关键词“GGA1 antibody N-terminal”或“GGA1 N-term epitope”检索获取。推荐查阅Juan S. Bonifacino、E.C. Dell'Angelica或Takashi Shiba等团队的研究。
The GGA1 (N-term) antibody is a specialized tool used to detect the N-terminal region of the Golgi-localized, γ-ear-containing ARF-binding protein 1 (GGA1), a critical adaptor protein involved in intracellular vesicular trafficking. GGA proteins, including GGA1. GGA2. and GGA3. regulate cargo sorting between the trans-Golgi network (TGN) and endosomes by interacting with clathrin, ARF GTPases, and cargo receptors such as mannose-6-phosphate receptors. The N-terminal region of GGA1 contains a VHS domain (VPS27. Hrs, STAM), which recognizes specific sorting signals on cargo proteins, and a GAT domain (GGA and TOM1) that binds ubiquitin and ARF proteins, facilitating membrane recruitment.
This antibody is commonly used in research to study GGA1's role in protein trafficking, lysosomal biogenesis, and receptor recycling. It helps visualize endogenous or overexpressed GGA1 via techniques like Western blotting, immunofluorescence, or immunoprecipitation, aiding investigations into its spatial-temporal regulation in cellular processes. Dysregulation of GGA1 has been implicated in neurodegenerative diseases (e.g., Alzheimer’s) due to its interaction with amyloid precursor protein (APP) trafficking. The N-term specificity ensures recognition of full-length GGA1. distinguishing it from potential cleavage products or isoforms. Validation often includes knockout controls to confirm target specificity.
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