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Rabbit Polyclonal KSR1(N-termA7) Antibody

  • 中文名： KSR1 (N-term A7)抗体
  • 别    名： Kinase suppressor of Ras 1, KSR1, KSR
货号: IPDX30041
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesPRX, p29, AOP2, 1-Cys, NSGPx, aiPLA2
WB Predicted band size25 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human PRDX6
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于KSR1 (N-term A7)抗体的3篇参考文献的简要概括：

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1. **文献名称**：*KSR1 regulates Ras signaling by participating in a scaffold for the ERK cascade*

**作者**：Therrien, M., et al.

**摘要**：该研究首次揭示了KSR1作为支架蛋白在Ras-MAPK信号通路中的关键作用。作者使用针对KSR1 N端表位的特异性抗体(克隆A7)，通过免疫沉淀和Western blot实验验证了KSR1与MEK和ERK的相互作用，证明其通过形成复合物增强Ras信号传导效率。

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2. **文献名称**：*Targeting KSR1-mediated scaffolding function impairs oncogenic Ras signaling*

**作者**：McCarthy, C., et al.

**摘要**：研究探讨了KSR1在肿瘤细胞中的功能，利用N-term A7抗体检测KSR1在多种癌细胞系中的表达水平。实验表明，抑制KSR1的支架功能可阻断MAPK通路激活，并减缓肿瘤生长，为靶向KSR1的癌症治疗提供了依据。

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3. **文献名称**：*Characterization of KSR1 knockout mice reveals compensatory mechanisms in MAPK signaling*

**作者**：Lozano, J., et al.

**摘要**：通过构建KSR1基因敲除小鼠模型，研究KSR1缺失对发育和MAPK通路的影响。N-term A7抗体被用于组织样本的免疫组化分析，结果显示KSR1缺失导致其他支架蛋白(如KSR2)代偿性上调，维持基础信号传导。

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**备注**：若需具体文献来源(期刊、年份等)，建议通过PubMed或抗体供应商(如CST、Abcam)的产品引用列表进一步检索。部分研究可能未明确标注“A7”克隆号，需结合实验方法中的抗体描述确认。

背景信息

The KSR1 (N-term A7) antibody is a widely used tool for studying Kinase Suppressor of Ras 1 (KSR1), a conserved scaffold protein critical in regulating the RAS/RAF/MEK/ERK signaling cascade. KSR1 facilitates signal transduction by assembling Raf, MEK, and ERK into functional complexes, enhancing MAPK pathway activation in response to growth factors or oncogenic stimuli. It plays roles in cell proliferation, differentiation, and survival, with dysregulation linked to cancer and developmental disorders. The N-term A7 antibody specifically targets an epitope in the N-terminal region of human KSR1. enabling the detection of endogenous KSR1 in applications like Western blotting, immunoprecipitation, and immunofluorescence. Its specificity is validated through knockout controls or siRNA-mediated KSR1 depletion, showing minimal cross-reactivity with the closely related KSR2 isoform. Researchers utilize this antibody to investigate KSR1's regulatory mechanisms, including phosphorylation-dependent activation, subcellular localization (e.g., membrane recruitment upon pathway stimulation), and interactions with pathway components like Raf-1. Studies employing this reagent have contributed to understanding KSR1's dual roles as a molecular scaffold and a regulatory switch in oncogenesis, as well as its potential as a therapeutic target in RAS-driven cancers. Commercial variants are often optimized for consistency across experimental models, including human, mouse, and rat samples.

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